Description
Natural Killer Cells, 1st ed. 2016
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1441
Coordinator: Somanchi Srinivas S.
Language: EnglishSubject for Natural Killer Cells:
Support: Print on demand
365 p. · 17.8x25.4 cm · Hardback
Description
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This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as ex vivo expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors, electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, Natural Killer Cells: Methods and Protocols is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.
1. Tracking Effector and Memory NK Cells During MCMV Infection
Aimee M. Beaulieu and Joseph C. Sun
2. Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells
Alexander W. Kay, Dara M. Strauss-Albee, and Catherine A. Blish
4. Genotyping Single Nucleotide Polymorphisms and Copy Number Variability of the FCGRs Expressed on NK Cells
Amy K. Erbe, Wei Wang, Mikayla Gallenberger, Jacquelyn A. Hank, and Paul M. Sondel
Sourindra N. Maiti
Karin Tomin, Ronald H. Goldfarb, and Per Albertsson
Saravanan Nandagopal, Francis Lin, and Sam K.P. Kung
Bruno Vanherberghen, Thomas Frisk, Elin Forslund, Per E. Olofsson, Karolin Guldevall, and Björn Önfelt
Kelsey J. McCulleyand Srinivas S. Somanchi
Jakob Theorelland Yenan T. Bryceson
Emily M. Mace and Jordan S. Orange
Grant Bertolet and Dongfang Liu
15. Ex-Vivo Expansion of Human NK Cells Using K562 Engineered to Express Membrane Bound IL21
16. Large-Scale Culture and Genetic Modification of Human Natural Killer Cells for Cellular Therapy
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras