Natural Products From Marine Algae, 2015
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1308

This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing methodologies. Natural Products from Marine Algae: Methods and Protocols guides readers through protocols and techniques on algal biotechnology, metabolites, Solid-Liquid Extraction (SLE), Microwave Assisted Extraction (MAE), Liquid Chromatography, Gas Chromatography, Nuclear Magnetic Resonance Spectroscopy, Infra-red spectroscopy and Raman Spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Natural Products from Marine Algae: Methods and Protocols hopes to aid scientists unravel and quantify algal chemical diversity and support further marine biotechnological developments.

Marine Algae a Source Of Biomass for Biotechnological Applications.- Structure and Function of Macroalgal Natural Products.- Spectrophotometric Assays of Major Compounds Extracted From Algae.- Extraction and Enrichment of Protein From Red and Green Macroalgae.- Extraction and Purification of R-phycoerythrin from Marine Red Algae.- Extraction and Analysis of Mycosporine-like Amino Acids in Marine Algae.- Extraction and Purification Of Phlorotannins From Brown Algae.- Enzyme-enhanced Extraction Of Antioxidant Ingredients From Algae.- Microwave-assisted Extraction Of Fucoidan From Marine Algae.- Extraction and Analysis of Oxylipins from Macroalgae Illustrated on the Example Gracilaria vermiculophylla.- Lipids and Fatty Acids In Algae: Extraction, Fractionation Into Lipid Classes and Analysis by Gas Chromatography Coupled with Flame Ionisation Detector (GC-FID).- HRMAS NMR Analysis Of Algae and Identification of Molecules of Interest Via Conventional 1D and 2D NMR: Sample Preparation and Optimization Of Experimental Conditions.- Extraction, Purification and NMR Analysis Of Terpenes From Brown Algae.- Extraction, Isolation and Identification of Sesquiterpenes From Laurencia Species.- The use of HPLC for the Characterisation Of Phytoplankton Pigments.- Characterization of Phlorotannins from Brown Algae by LC-HRMS.- Analysis of Betaines From Marine Algae using LC-MS-MS.- Analysis of Marine Biotoxins using LC-MS/MS.- Fucoidan Analysis by Tandem MALDI-TOF and ESI Mass Spectrometry.- Determination of Substitution Patterns of Galactans from Green Seaweeds Of The Bryopsidales.- Structural Characterization Of A Hybrid Carrageenan-Like Sulphated Galactan from a Marine Red Alga Furcellaria lumbricalis.- Characterisation Of Alginates By Nuclear Magnetic Resonance (NMR) and Vibrational Spectroscopy (IR, NIR, Raman) in Combination with Chemometrics.- Imaging and Identification of Marine Algal Bio-active Compounds by Surface Enhanced Raman Spectroscopy (S

ERS).- In vitro protocols for Measuring the Antioxidant Capacity of Algal Extracts.- Disk Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts.- Screening of a Marine Algal Extract for Antifungal Activities.- Protocol for Assessing Antifouling Activities of Macroalgal Extracts.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts