Description
Nucleic Acid Detection, Softcover reprint of the original 1st ed. 2013
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1039
Coordinators: Kolpashchikov Dmitry M., Gerasimova Yulia V.
Language: EnglishKeywords
DNA; DNA for amplification and analysis; RNA; RNA monitoring in live cells; capillary electrophoresis; fluorescent in situ hybridization; isothermal amplification techniques; microarrays; molecular beacon probes for micro RNA analysis; nucleic acid detection methods; point-of care diagnostics; qPCR chemistries; signal amplification technique; visual or electric signals
Publication date: 08-2016
Support: Print on demand
Publication date: 09-2013
320 p. · 17.8x25.4 cm · Hardback
Description
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In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.
Part I: Advances in Sample Preparation
1. “Getting Blood from a Stone”: Ultra-Sensitive Forensic DNA Profiling of Microscopic Bio-particles Recovered from ‘Touch DNA’ Evidence
Erin K. Hanson and Jack Ballantyne
2. Detecting MicroRNA in Human Cancer Tissues with Fluorescence in situ Hybridization
Zonggao Shi, Jeff J. Johnson, and M. Sharon Stack
Part II: New Real-Time and Instantaneous Assays
3. MN Azyme qPCR: A Superior Tool for Multiplex qPCR
Elisa Mokay and Alison V. Todd
4. A New, Multiplex, Quantitative Real-time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification
Fang Liang, Neetika Arora, Kang Liang Zhang, David Che Cheng Yeh, Richard Lai, Darnley Pearson, Graeme Barnett, David Whiley, Theo Sloots, Simon R. Corrie, and Ross T. Barnard
5. Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with Universal Molecular Beacon Reporter
Yulia V. Gerasimova, Jack Ballantyne, and Dmitry M. Kolpashchikov
6. SNP Analysis using a Molecular Beacon-based Operating Cooperatively (OC) Sensor
Evan M. Cornett and Dmitry M. Kolpashchikov
Part III: Isothermal Amplification Methods for Nucleic Acid Detection
7. Detection of rpoB Gene Mutations Using Helicase-Dependent Amplification
Wanyuan Ao and Robert Jenison
8. Rapid Detection of Brucella spp. using Loop-mediated Isothermal Amplification (LAMP)
Shouyi Chen, Xunde Li, Juntao Li, and Edward R. Atwill
9. Loop-mediated Isothermal Amplification Method for a Differential Identification of Human Taenia Tapeworms
Yasuhito Sako, Agathe Nkouawa, Tetsuya Yanagida, and Akira Ito
10. Detection of Mutation by Allele-specific Loop-mediated Isothermal Amplification (AS-LAMP)
Hiroka Aonuma, Athanase Badolo , Kiyoshi Okado, and Hirotaka Kanuka
Part IV: Signal Amplification Approac
11. DNA Detection by Cascade Enzymatic Signal Amplification
Bingjie Zou, Yinjiao Ma and Guohua Zhou
Part V: Non-Fluorescent Detection Formats
12. Visual DNA Detection and SNP Genotyping using Asymmetric PCR and Split DNA Enzymes
Jia Ling Neo and Mahesh Uttamchandani
13. Detection of Single-stranded Nucleic Acids via Colorimetric Means, using G-quadruplex Probes
Shizuka Nakayama and Herman O. Sintim
14. Lateral Flow Biosensors for the Detection of Nucleic Acid
Lingwen Zeng, Puchang Lie, Zhiyuan Fang, Zhuo Xiao
15. Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a
Wireless Bipolar Electrode
Mei-Sheng Wu, Guang-sheng Qian, Jing-Juan Xu, and Hong-Yuan Chen
16. High-throughput Label-free Detection of DNA Hybridization and Mismatch Discrimination Using Interferometric Reflectance Imaging Sensor
Sunmin Ahn, David S. Freedman, Xirui Zhang, and M. Selim Ünlü1
Part VI: Advances in Fish
17. Graphene -PAMAM Dendrimer -Gold Nano Particle Composite for Electrochemical DNA Hybridization Detection
Kumarasamy Jayakumar, Rajendiran Rajesh ,Venkataraman Dharuman, and Rangarajan Venkatesan
18. Application of PNA Openers for Fluorescence-based Detection of Bacterial DNA
Irina Smolina
19. DNA probes for FISH analysis of C-Negative Regions in Human Chromosomes
Evgeniy S. Morozkin, Tatyana V. Karamysheva, Pavel P. Laktionov, Valentin V. Vlassov, and Nikolay B. Rubtsov
Part VII: Detection of microRNA
20. Quantitative Analysis of MicroRNA in Blood Serum with Protein-Facilitated Affinity Capillary Electrophoresis
Nasrin Khan and Maxim V. Berezovski
21. High-Throughput Functional MicroRNA Profiling using Recombinant AAV-Based MicroRNA Sensor Arrays
Wenhong Tian, Xiaoyan Dong, Xiaobing Wu, and Zhijian Wu
22. The Use of Molecular Beacons to Detect and Quantify MicroRNA
Meredith B. Baker, Gang Bao, and Charles D. Searles
Part VIII: RNA Imaging in Live Cells
23. Sequence Specific Imaging of Influenza A mRNA in Living Infected Cells Using Fluorescent FIT-PNA
Susann Kummer, Andrea Knoll, Andreas Herrmann, and Oliver Seitz
24. Application of Caged Fluorescent Nucleotides to Live-cell RNA Imaging
Akimitsu Okamoto
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras