Proteases in Apoptosis: Pathways, Protocols and Translational Advances, 1st ed. 2015

This book provides a comprehensive overview of the proteases involved in programmed cell death. It presents a focused yet extensive discussion on proteolytic enzymes such as caspases, HtrAs, granzymes, calpains and cathepsins as well as laboratory protocols related to enzymology and apoptosis. Mouse model systems and non-invasive imaging techniques in apoptosis-related diseases such as cancer and neurodegeneration are also covered in this book.

While slowly unravelling the complexities of apoptosis in chapter one,the next three chapters individually elaborate on different classes of proteases that play key roles in the initiation, progression and execution of programmed cell death. The last two chapters complete this discussion by describing different laboratory methodologies and therapeutic advances involving apoptotic proteases. Protocols portraying in vitro and ex vivo colorimetric and fluorescence-based enzyme kinetic studies as well as cell death assays are explained in the fifth chapter. Preclinical in vivo models and non-invasive imaging in apoptosis to understand the complexities of disease progression and their contribution toward therapeutics is recounted in the last chapter.

The book spans topics related to both fundamental and applied biology. It would therefore be equally appealing and informative to scientists working in the field of apoptosis and those who are investigating mechanisms of proteases and enzymes in general. The protocols would certainly benefit both graduate and undergraduate students working in the related fields and provide useful leads for drug design to translational biologists involved in neurodegeneration and cancer research.

Preface

1: Apoptosis: Pathways, Molecules and Beyond                                                    

2: Caspases - key players in apoptosis

3: Calpains and Granzymes: non-caspase proteases in cell death                                               

4: Cathepsins and HtrAs - multitasking proteases in programmed cell death

5: Proteases in Apoptosis: Protocols and Methods

6: Preclinical Animal Model and Non-invasive Imaging in Apoptosis

Index

Gives fundamental and exclusive information on all proteases involved in programmed cell death in a single compilation

Is an essential source of information in any biomedical scientist's library

Provides step-by-step protocols on biochemical, cell biology, in vivo and non invasive studies involving diseases associated with the proteases in apoptosis

Shows unique information on the different approaches to target these proteases for disease intervention such as neurodegeneration and cancer

Includes supplementary material: sn.pub/extras