Description
Resin Microscopy and On-Section Immunocytochemistry (2nd Ed., 2nd ed. 2001)
Springer Lab Manuals Series
Authors: Newman Geoffrey R., Hobot Jan A.
Language: EnglishSubjects for Resin Microscopy and On-Section Immunocytochemistry:
158.24 €
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Resin Microscopy and On-Section Immunocytochemistry (2nd Ed.)
Publication date: 08-2014
273 p. · 19.3x24.2 cm · Paperback
Publication date: 08-2014
273 p. · 19.3x24.2 cm · Paperback
210.99 €
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Add to cart the print on demand of Newman Geoffrey R., Hobot Jan A.
Resin microscopy and on-section immunocytochemistry (2nd Ed.)
Publication date: 04-2001
Support: Print on demand
Publication date: 04-2001
Support: Print on demand
Description
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Since antibodies tagged with markers have been developed, immunocytochemistry has become the method of choice for identifying tissue substances or for the localisation of nucleic acid in tissue by in situ hybridisation. Resin-embedded tissue is routinely used and new techniques are constantly introduced. Thus, the novice entering these fields has a breathtaking variety of methods open to him. This labmanual covers the embedding of tissue using epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities and results are discussed so that an understanding of the techniques can be acquired and appropriate choices made. The various resins available and all steps involved in tissue processing, beginning with fixation, as well as the great variety of labelling methods and markers that are commonly used for "on-section" cytochemistry and immunocytochemistry are described, including detailed protocols for the application.
I: Resin Embedding.- 1 The Strategic Approach.- 2 The Resins.- 3 Resin Embedding Protocols for Chemically Fixed Tissue.- 4 Cryotechniques.- 5 Methods for Resin Polymerisation.- 6 Handling Resin Blocks.- II: On-Section Immunolabelling.- 7 Strategies in Immunolabelling.- 8 General Considerations.- 9 Immunolabelling Protocols for Resin Sections.- 10 Resin Embedding and Immunolabelling.- Appendix I Examples of Typical Resin Embedding Regimes for Immunocytochemistry.- I.1 Solid Tissue, Pellets and Agar Blocks — Immersion Fixation.- I.2 Solid Tissue — Light Perfusion Fixation.- I.3 Solid Tissue — Very Light Perfusion Fixation.- I.4 Cryosubstitution.- I.5 Project Planner.- Appendix II List of Suppliers.- EM General.- EM Apparatus.- Electron Microscopes.- Flow Cytometry.- References.
Ready-to-go protocols Explains all steps from fixation to labelling and detection Helps in finding the resin of choice for your needs
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