Sphingosine-1-Phosphate, Softcover reprint of the original 1st ed. 2012
Methods and Protocols
Methods in Molecular Biology Series, Vol. 874

Sphingosine-1-phosphate is a bioactive lysophospholipid which has become, in recent years, the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.  In Sphingosine-1-Phosphate: Methods and Protocols, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology.  As a volume in the Methods in Molecular Biology? series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

1. Biochemical Methods for Quantifying Sphingolipids: Ceramide, Sphingosine, Sphingosine Kinase-1 Activity, and Sphingosine-1-Phosphate

            Brizuela Leyre and Cuvillier Olivier

2. Isoform-Selective Assays for Sphingosine Kinase Activity

            Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson

3. Quantification of Sphingosine-1-Phosphate and Related Sphingolipids by Liquid Chromatography Coupled to Tandem Mass Spectrometry

            Constantin Bode and Markus H. Gräler

4. A Clean-up Method for Mass Spectrometric Analysis of Sphingosine-1-Phosphate in Blood and Solid Tissues Using a Phosphate Capture Molecule

            Jun-ichi Morishige, Tamotsu Tanaka, and Kiyoshi Satouchi

5. Immunohistochemical Detection of Sphingosine-1-Phosphate and Sphingosine Kinase-1 in Human Tissue Samples

            Barbara Visentin, Gary Reynolds, and Roger Sabbadini

6. Assessment of Sphingosine-1-Phosphate Activity in Biological Samples by Receptor Internalization and Adherens Junction Formation

            Hideru Obinata and Timothy Hla

7. High Throughput Assays to Measure Intracellular Ca²⁺ Mobilization in Cells that Express Recombinant S1P Receptor Subtypes

            William J. Valentine and Gabor Tigyi

8. Quantifying Sphingosine-1-Phosphate-Dependent Activation of the RhoGTPases

            Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack

9. Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal Exocytosis in Human Sperm

            Silvia A. Belmonte and Laila Suhaiman

10. Use of Intravital Microscopy and In Vitro Chemotaxis Assays to Study the Roles of Sphingosine-1-Phosphate in Bone Homeostasis

            Taeko Ishii, Shunsuke Kawamura, Issei Nishiyama, Junichi Kikuta, and Masaru Ishii

11. Assessment of Sphingosine-1-Phosphate Receptor Expression and Associated Intracellular Signaling Cascades in Primary Cells of the Human Central Nervous System

            Veronique E. Miron, Bryce A. Durafourt, Jack P. Antel, and Timothy E. Kennedy

12. Sphingosine-1-Phosphate Signaling in Skeletal Muscle Cells

            Elisabetta Meacci, Francesca Bini, and Chiara Battistini

13. Maintenance of Human Embryonic Stem Cells by Sphingosine-1-Phosphate and Platelet-Derived Growth Factor

            Raymond C.B. Wong, Martin F. Pera, and Alice Pébay

14. Ceramide and Sphingosine-1-Phosphate Signaling in Embryonic Stem Cell Differentiation

            Erhard Bieberich

15. Sphingosine-1-Phosphate: An Alternative to Serum for Culture and Multipotency Maintenance of Adult Human Mesenchymal Stem Cells

            He Xiaoli, Sathish Jean, and Alirio J. Melendez

16. Sphingosine-1-Phosphate Signaling in Neural Progenitors

            Phillip Callihan and Shelley B. Hooks

17.Utilizing Sphingosine-1-Phosphate to Stimulate Sprouting Angiogenesis

            Shih-Chi Su and Kayla J. Bayless

Features the important effects of S1P in cell development and physiopathology

Includes detailed, tried-and-true protocols, ready for the lab

Contains expert tips and key troubleshooting advice to ensure successful results

Includes supplementary material: sn.pub/extras