Standardisation in Cell and Tissue Engineering
Methods and Protocols
Woodhead Publishing Series in Biomaterials Series

Coordinator: Salih V

Language: English
Cover of the book Standardisation in Cell and Tissue Engineering

Subjects for Standardisation in Cell and Tissue Engineering

Publication date:
276 p. · 15.5x23.3 cm · Hardback
Out of Print
The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules, in order to create functional replacement tissue in a damaged tissue site, has led to the need for the development of ?best practice? methods in the area of tissue engineering to help ensure the creation of safe, high quality products. Standardisation in cell and tissue engineering introduces concepts and current practice in the field of cell and tissue engineering to a wide audience and aims to provide awareness of the importance of standardisation in this area while suggesting directions for further investigation.

Part one provides an overview of methods for cell and tissue engineering and includes chapters on the fundamentals of cell and matrix biology for tissue engineering, 3D collagen biomatrix development, and control and vascularisation of tissue-engineered constructs. Part two begins with a chapter exploring the methods and protocols of standardisation in cell and tissue engineering before moving on to highlight issues of quality control in cell and tissue engineering, standardised chemical analysis and testing of biomaterials and principles of good laboratory practice (GLP) for in vitro cell culture applications.

Standardisation in cell and tissue engineering is a standard reference for leading research groups, government agencies, regulatory bodies, and researchers and technicians at all levels across the whole range of disciplines using cell culture within the pharmaceutical, biotechnology and biomedical industries.

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Woodhead Publishing Series in Biomaterials

Foreword

Introduction

Part I: Methods for cell and tissue engineering

Chapter 1: Fundamentals of cell and matrix biology for tissue engineering

Abstract:

1.1 Introduction

1.2 Extracellular matrices (ECMs)

1.3 ECM and cell interaction

1.4 ECM and mechanical signalling

1.5 Future trends

1.6 Conclusion

Chapter 2: Three-dimensional collagen biomatrix development and control

Abstract:

2.1 Engineering cell-rich and matrix-rich tissues using collagen scaffolds

2.2 Controlling the mechanical properties of collagen

2.3 Architectural features: introducing elements of tissue complexity

2.4 Future trends

Chapter 3: Two- and three-dimensional tissue culture bioprocessing methods for soft tissue engineering

Abstract:

3.1 Introduction

3.2 Bioreactor configurations

3.3 Selecting scaffold materials and architectures for your bioreactor

3.4 Mass transfer in tissue engineering bioreactors

3.5 Important parameters and taking measurements of bioreactor cultures

3.6 Tissue engineering process design

3.7 Future trends

3.8 Conclusion

3.9 Sources of further information and advice

Chapter 4: Two- and three-dimensional tissue culture methods for hard tissue engineering

Abstract:

4.1 Introduction

4.2 Culture of bone and cartilage cells

4.3 Cell culture parameters: bone tissue culture

4.4 Cell culture parameters: cartilage tissue culture

4.5 Two-dimensional tissue culture methods for hard tissues

4.6 Two-and-a-half- and three-dimensional tissue culture methods for hard tissues

4.7 Conclusion

Chapter 5: Vascularisation of tissue-engineered constructs

Abstract:

5.1 Introduction

5.2 Growth of healthy vessels ‒ embryonic vasculogenesis

5.3 Angiogenic diseases

5.4 Angiogenesis and bone formation

5.5 Cell sources for vascular tissue engineering

5.6 Co-culture of cells: the interactions between angiogenesis and osteogenesis

5.7 Strategies to induce in vitro prevascularisation

5.8 Tubular formation

5.9 Conclusion

Part II: Standards and protocols in cell and tissue engineering

Chapter 6: Standards in cell and tissue engineering

Abstract:

6.1 Introduction

6.2 How and by whom are standards produced?

6.3 The importance of an agreed lexicon

6.4 Drivers for standardization

6.5 How will standards help me?

6.6 What standards currently exist in tissue engineering?

6.7 Characterization of biomaterials and biomolecules

6.8 Characterization of tissue scaffolds

6.9 Characterization of cell-seeded scaffolds

6.10 Manufacture, processing and storage

6.11 Characterization of cells and cell-surface interactions

6.12 Conclusion

Chapter 7: Principles of good laboratory practice (GLP) for in vitro cell culture applications

Abstract:

7.1 Introduction

7.2 GLP governing bodies

7.3 Resources required for GLP compliance

7.4 Characterisation

7.5 Standards and regulations

7.6 Documentation of results

7.7 Independent monitoring of research processes and quality assurance (QA) personnel

7.8 Application of GLP to human cell culture systems

7.9 Conclusion

7.10 Acknowledgements

Chapter 8: Quality control in cell and tissue engineering

Abstract:

8.1 Introduction

8.2 Quality control to ensure a well-defined cell therapy product

8.3 Commercial quality control/quality assurance in large-scale manufacture

8.4 Conclusion

Chapter 9: Standardised chemical analysis and testing of biomaterials

Abstract:

9.1 Introduction: why we need standard methods for testing biomaterials

9.2 Standardised chemical analysis: when and why we assess chemistries

9.3 Chemical properties

9.4 Imaging methods for measuring porosity

9.5 Physical characterisation – permeability

9.6 Surface properties

9.7 Degradation and stability in physiological fluids

9.8 Implant–tissue interface tests

9.9 Limitations of current standardised testing methods

Chapter 10: Sterilisation procedures for tissue allografts

Abstract:

10.1 Introduction

10.2 Interaction of ionising radiation with matter

10.3 Sources of ionising radiation

10.4 Validation and international standards of sterilisation by ionising radiation

10.5 Conclusions and future trends

10.6 Sources of further information and advice

Chapter 11: Commercial manufacture of cell therapies

Abstract:

11.1 Introduction: cells as therapies

11.2 The transition from laboratory to commercial- scale manufacture of cell therapies

11.3 Key regulatory requirements for commercial manufacture of cell therapies

11.4 Cell-based therapy versus monoclonal antibody therapies: lessons from existing biopharmaceutical manufacture

11.5 Conclusion

Index

Dr Vehid Salih is Reader at Peninsula Dental School, Plymouth University Schools of Medicine and Dentistry, UK.
  • Introduces concepts and current practice in the field of cell and tissue engineering
  • Highlights the importance of standardisation in cell and tissue engineering and suggests directions for further investigation
  • Explores methods and protocols of standardisation in cell and tissue engineering and issues of quality control in cell and tissue engineering