Transgenic Cotton, Softcover reprint of the original 1st ed. 2013
Methods and Protocols
Methods in Molecular Biology Series, Vol. 958

Cotton is the most important textile and cash crop and is widely cultivated in more than 70 countries, including the United States, China, and India. Because its long life cycle and complicated genetic background, it is hard to improve cotton using traditional breeding techniques although it has made much progress in the last several decades. Currently, transgenic techniques have become a powerful tool to improve cotton and transgenic cotton is among the first commercially genetically modified crops. Transgenic Cotton: Methods and Protocols provides a comprehensive collection of methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Divided into five convenient sections, topics covered include the current status and perspectives of transgenic cotton, the principle and methods for making transgenic cotton, the methods for detecting foreign gene copy and expression in transgenic plants, the improvement of cotton using transgenic technology, and finally the methods for monitoring the potential impact of transgenic cotton on environment, including gene flow. Written in the successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Transgenic Cotton: Methods and Protocols will serve as an excellent resource for scientists as well as graduate students who work on transgenic plants, plant genetics, molecular biology and agricultural sciences.

Transgenic Cotton: From Biotransformation Methods to Agricultural Application.- Genetically Modified Cotton in India and Detection Strategies.- Agrobacterium-Mediated Transformation of Cotton.- Biolistic Transformation of Cotton Zygotic Embryo Meristem.- Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures.- Cotton Transformation via Pollen Tube Pathway.- Silicon Carbide Whisker-Mediated Transformation of Cotton (Gossypium hirsutum L.).- Investigating Transgene Integration and Organization in Cotton (Gossypium hirsutum L.) Genome.- Estimating the Copy Number of Transgenes in Transformed Cotton by Real-Time Quantitative PCR.- Development of Enzyme-Linked Immunosorbant (ELISA) Assay for the Detection of Bt Protein in Transgenic Cotton.- DNA-Based Diagnostics for Genetically Modified Cotton: Decaplex PCR Assay to Differentiate MON531 and MON15985 Bt Cotton Events.- A Simple and Rapid Method for Determining Transgenic Cotton Plants.- An Efficient Grafting Technique for Recovery of Transgenic Cotton Plants.- Inheritance of Transgenes in Transgenic Bt Lines Resistance to Helicoerpa armigera in Upland Cotton.- Agrobacterium rhizogenes-Induced Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics.- Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation.- Development of Transgenic CryIA(c)+GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover.- Agrobacterium-Mediated Transformation of Cotton (Gossypium hirsutum) Shoot Apex with a Fungal Phytase Gene Improves Phosphorus Acquisition.- Genetic Transformation of Cotton with a Harpin-Encoding Gene hpaXoo Confers an Enhanced Defense Response Against Verticillium dahliae Kleb.- Development of Insect-Resistant Transgenic Cotton with Chimeric TVip3A* Accumulating in Chloroplasts.- Determining Gene Flow in Transgenic Cotton.