Wound Healing, 2003
Methods and Protocols
Coll. Methods in Molecular Medicine, Vol. 78

During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess. Many of these new techniques have been utilized in the context of more classic models of wound healing. The combination of new and classic approaches has allowed scientists to make exciting discoveries in the field of tissue repair, resulting in an explosion of information about the healing p- cess. Importantly, these new findings have great relevance beyond wound healing itself. The injury repair process cuts across many disciplines, exte- ing to such broad fields as cancer, inflammation, and atherosclerosis. The relevance of the field to these many disciplines has generated great interest in models and methods for the study of wound healing. The goal of Wound Healing: Methods and Protocols is to provide scientists from many dis- plines with a compendium of classic and contemporary protocols from r- ognized experts in the field of wound healing. We hope this volume will be useful not only to those working within the field itself, but also to scientists from other disciplines who wish to adapt wound healing models to their own experimental needs. The process of wound healing encompasses many different biologic p- cesses, including epithelial growth and differentiation, fibrous tissue prod- tion and function, angiogenesis, and inflammation.

Experimental Models of Wound Healing.- Excisional Wound Healing.- Methods in Reepithelialization.- Incisional Wound Healing.- Animal Models of Ischemic Wound Healing.- Corneal Injury.- Subcutaneous Sponge Models.- A Mouse Model of Burn Wounding and Sepsis.- A Porcine Burn Model.- Wound Healing in Airways In Vivo.- Murine Models of Intestinal Anastomoses.- Murine Model of Peritoneal Adhesion Formation.- Methods for Investigating Fetal Tissue Repair.- Growth of Human Blood Vessels in Severe Combined Immunodeficient Mice.- Tissue Repair in Models of Diabetes Mellitus.- Wound Healing Studies in Transgenic and Knockout Mice.- Wound Repair in Aging.- Specimen Collection and Analysis.- Suction Blister Model of Wound Healing.- Implantable Wound Healing Models and the Determination of Subcutaneous Collagen Deposition in Expanded Polytetrafluoroethylene Implants.- The Fibroblast-Populated Collagen Lattice.- A Quantifiable In Vitro Model to Assess Effects of PAI-1 Gene Targeting on Epithelial Cell Motility.- Human Skin Organ Culture.- In Vitro Matrigel Angiogenesis Model.- Analysis and Manipulation of Wound Healing.- Quantification of Wound Angiogenesis.- In Vivo Matrigel Migration and Angiogenesis Assays.- Endothelial Cell Migration Assay.- Analysis of Collagen Synthesis.- Method for Detection and Quantitation of Leukocytes During Wound Healing.- Detection of Reactive Oxygen Intermediate Production by Macrophages.- Measurement of Chemokines at the Protein Level in Tissue.- Methods of Measuring Oxygen in Wounds.- Isolation, Culture, and Characterization of Human Intestinal Smooth Muscle Cells.- Use of High-Throughput Microarray Membranes for cDNA Analysis of Cutaneous Wound Repair.- Particle-Mediated Gene Therapy of Wounds.

Includes supplementary material: sn.pub/extras