Description
Fluorescence Lifetime Spectroscopy and Imaging
Principles and Applications in Biomedical Diagnostics
Coordinators: Marcu Laura, French Paul M. W., Elson Daniel S.
Language: EnglishSubjects for Fluorescence Lifetime Spectroscopy and Imaging:
Keywords
Fluorescence Lifetime; Uo Rescencelifetim; autofluorescence; Fluorescence Decay; FLIM; Fluorescence Lifetime Imaging; Im Ag; spectroscopy; Fluorescence Lifetime Measurements; global fitting; Endogenous Fluorophores; time-resolved fluorescence; Fluorescence Decay Profiles; abel-free diagnosis; cancer; Ffl Uo; cardiovascular disease; FLIM Image; ophthalmology; Brain Tumor; FLIM Data; De Beule; Frequency Domain FLIM; FLIM Measurement; Wide Field FLIM; Endogenous Fluorescence; FLIM System; Lifetime Imaging; Spectral Channels; Average Fluorescence Lifetime; Lifetime Components; Decay Profiles; Multiphoton Imaging
Publication date: 06-2020
· 17.8x25.4 cm · Paperback
Publication date: 07-2014
554 p. · 17.8x25.4 cm · Hardback
Description
/li>Contents
/li>Biography
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During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications.
Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation.
Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.
Introduction. Overview of Fluorescence Measurements and Concepts. Principles of Fluorescence Lifetime Instrumentation. Analysis of Fluorescence Lifetime Data. Tissue Fluorescence Lifetime Spectroscopy. Tissue Fluorescence Lifetime Imaging (Endogenous). Fluorescence Lifetime Imaging (Exogenous Probes).
Laura Marcu, Paul M. W. French, Daniel S. Elson