Description
Antiviral Resistance in Plants, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2028
Coordinators: Kobayashi Kappei, Nishiguchi Masamichi
Language: EnglishSubjects for Antiviral Resistance in Plants:
Support: Print on demand
292 p. · 17.8x25.4 cm · Hardback
Description
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Section 1. Analysis of natural virus resistance mechanisms
1 Identification and functional analysis of NB-LRR-type virus resistance genes
Overview and Functional analysis of candidate genes
2-4 Analysis of resistance signaling pathways and resistance mechanisms
2 Genetic analysis in Arabidopsis thaliana
3 VIGS analysis in Nicotiana benthamiana4 Resistance mechanisms
5 Identification and functional analysis of recessive virus resistance genes
Overview and analytical methods
6-7 Identification of non-conventional virus resistance genes
6 Screening in diverse plants7 Biochemical identification of virus resistance genes
8-9 Isolation and analysis of virus resistance breaking strains
8 Analysis of field isolates and Error-prone PCR-based mutagenesis
9 Forced evolution in plants and selection in grafted scions
10-12 Analysis of RNA silencing-mediated virus resistance
10 Analysis of small RNAs (small RNA gel blotting, Deep sequencing)11 Analysis of DNA methylation and histone modification in virus resistance
12 Functional analysis of RNA silencing machinery
Section 2. Engineered virus resistance and Virus vaccine: Design and Evaluation
13 RNA silencing-based virus resistance with viral RNA and DNA targets
14 Virus resistance by CRISPR/Cas9 targeting viral sequences15 Resistance breeding through the control of host factors involved in virus replication
16 Virus resistance breeding through CRISPR/Cas9-mediated genome editing
17 Attenuated viruses: Isolation, evaluation and mechanisms
18 RNA silencing-mediated virus vaccine
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts