Doubled Haploid Technology, 1st ed. 2021 Volume 3: Emerging Tools, Cucurbits, Trees, Other Species Methods in Molecular Biology Series, Vol. 2289

This title offers 62 chapters divided among three volumes covering the latest topics dealing with Doubled Haploid (DH) technology, as well as methods to produce DHs in different species through different in vivo and in vitro approaches. Volume 3 looks at the emerging tools used in DH technology; protocols based on anther culture, in vitro gynogenesis and hybridization with irradiated pollen for DH production in cucubits such as cucumber, melon, watermelon, and summer squash; techniques to produce haploids and DHs in different woody species and genera such as citrus, coconut, walnut, and almond; and methods for DH production in borage, Saintpaulia ionantha, cow cockle, and marigold. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Doubled Haploid Technology, Volume 3: Emerging Tools, Cucurbits, Trees, Other Species is a valuable resource for scientists and researchers looking to learn more about this interesting and developing field.

Preface…
Table of Contents…
Contributing Authors… 

Part I Emerging Tools in DH Technology

1. Centromere Engineering as an Emerging Tool for Haploid Plants Production: Advances and Challenges
Raheleh Karimi-Ashtiyani

2. Fractional Factorial Designs: An Underutilized Tool for Rapid In Vitro System Development
Randall P. Niedz

3. Applications of Impedance Flow Cytometry in Doubled Haploid Technology
Iris Heidmann and Marco Di Berardino

Part II Doubled Haploids in Cucurbits

4. Production of Doubled Haploid Plants in Cucumber (Cucumis sativus L.) through Anther Culture
Abouzar Asadi and Jose M. Seguí-Simarro

5. Doubled Haploid Parthenogenetic Production of Melon 'Piel de Sapo' 
Isidre d’Hooghvorst, Oscar Torrico, and Salvador Nogués

6. Doubled Haploid Production in Watermelon
Nebahat Sari and Ilknur Solmaz

7. Anther Culture in Cucurbita Species
Ertan Sait Kurtar and Musa Seymen

8. Gynogenesis in Cucurbita Species
Ertan Sait Kurtar and Musa Seymen

9. Induction of Parthenogenesis by Irradiated Pollen in Cucurbita Species
Ertan Sait Kurtar and Musa Seymen

Part III Doubled Haploids in Woody Species

10. Microspore Embryogenesis in Citrus
Emna Yahyaoui and Maria Antonietta Germanà

11. Anther Culture of Coconut (Cocos nucifera L.)
Prasanthi I. P. Perera and Vijitha R. M. Vidhanaarachchi

12. Production of Haploid and Doubled Haploid Lines in Nut Crops: Persian Walnut, Almond, and Hazelnut
Kourosh Vahdati, Mohammad Sadat-Hosseini, Pedro Martínez-Gómez, and Maria Antonietta Germanà

13. Production of Doubled Haploid Embryos from Cork Oak Anther Cultures by Antimitotic Agents and Temperature Stress
Beatriz Pintos, José Antonio Manzanera, and Arancha Gómez-Garay

14. Anther Culture in Jatropha curcas L. - A Tree Species
Savarimuthu Arockiasamy, Manoj Patil, Vijay Yepuri, Vineeta Shrivastava, Neeta Madan, Sainath Hadole, and Purbasha Sarkar

Part IV Doubled Haploids in Other Species

15. Haploid Plant Production in Borage (Borago officinalis L.) by Anther Culture
Mohammad Reza Abdollahi, Zahra Chardoli Eshaghi, and Jose M. Seguí-Simarro

16. Anther-Based Regeneration of African Violet (Saintpaulia ionantha)
Yuichi Uno and Masafumi Yagi

17. Doubled Haploidy for Cow Cockle (Saponaria Vaccaria L.)
Alison M.R. Ferrie and Zoë Ehlert

18. Production of Haploids and Doubled Haploids in Marigold (Tagetes spp.) using Anther Culture
Ravindra Kumar Kurimella, Reeta Bhatia, Kanwar Pal Singh, and Sapna Panwar

19. Anther Culture of Chickpea (Cicer arietinum L.)
Mohammad Reza Abdollahi and Jose M. Seguí-Simarro

20. Production of Homozygous Red Beet (Beta vulgaris L subsp. vulgaris) Plants by Ovule-Culture
Waldemar Kiszczak, Maria Burian, Urszula Kowalska, Krystyna Górecka, and Małgorzata Podwyszyńska

21. Production of Doubled Haploid Sugar Beet (Beta vulgaris L.) Plants through Gynogenesis
Songul Gurel, Arman Pazuki, Fatemeh Aflaki, and Ekrem Gurel

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains keynotes and implementation advice from the experts