Isolation of Plant Organelles and Structures, 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1511

Coordinators: Taylor Nicolas L., Millar A. Harvey

Language: English
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Isolation of Plant Organelles and Structures
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This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.

1. The Isolation of Plant Organelles and Structures in the Post-Genomics Era

            A. Harvey Millar and Nicolas L. Taylor

 

2. Approaches to Characterize Organelle, Compartment, or Structure Purity

            Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski

 

Part I: Isolation of Organelles and Organelle Compartments

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3. Isolation of Nuclei and Nucleoli

            Alison F. Pendle and Peter J. Shaw

 

4. Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts

            Úrsula Flores-Pérez and Paul Jarvis

 

5. Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit

            Cristina Barsan, Marcel Kuntz,and Jean-Claude Pech

 

6. Leucoplast Isolation and Subfractionation

            William C. Plaxton

 

7. Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes

            Owen Duncan, A. Harvey Millar, and Nicolas L. Taylor

 

8. Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane

            Sigrun Reumann and Piotr Lisik

 

9. Isolation of Vacuoles and the Tonoplast

            Jan Zouhar

 

10. Isolation of Endoplasmic Reticulum and Its Membrane

            G. Eric Schaller

 

11. Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings

            Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, and Joshua L. Heazlewood

 

12. Isolation of Autolysosomes from Tobacco BY-2 Cells

            Chihiro Takatsuka, Yuko Inoue, and Yuji Moriyasu

 

13. Isolation of Protein Storage Vacuoles and Their Membranes

            Tomoo Shimada and Ikuko Hara-Nishimura

 

Part II: Isolation of Other Structures and Compartments

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14. Isolation of the Cell Wall

            Hervé Canut, Cécile Albenne, and Elisabeth Jamet

 

15. Isolation of Plasmodesmata

            Christine Faulkner and Emmanuelle M.F. Bayer

 

16. Isolation of Plasma Membrane and Plasma Membrane Microdomains

            Anzu Minami, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura

 

17. Enrichment of the Plant Cytosolic Fraction

            Jeemeng Lao, Andreia M. Smith-Moritz, Jennifer C. Mortimer, and Joshua L. Heazlewood

 

18. Isolation of Apoplast

            Erik Andreasson, Kibrom B. Abreha, and Svante Resjö

 

19. Isolation of Cytosolic Ribosomes

            Hanna Klang Årstrand and Maria V. Turkina

 

20. Isolation of Plastid Ribosomes

            Kenichi Yamaguchi

 

21. Isolation of Mitochondrial Ribosomes

            Adam J. Carroll

 

22. Isolation of Microtubules and Microtubule-Associated Proteins

            Takahiro Hamada and Seiji Sonobe

 

23. Isolation of Actin and Actin Binding Proteins

            Etsuo Yokota

 

24. Purification of 26S Proteasomes and Their Sub-Complexes from Plants

            Richard S. Marshall, David C. Gemperline, and Richard D. Vierstra

Includes cutting-edge methods and protocols for the study of plant organelles and structures

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts