Plant Argonaute Proteins, Softcover reprint of the original 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1640

This volume provides the reader with step?by?step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO?small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non?radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.

1.        Plant ARGONAUTEs: Features, Functions and Unknowns

Alberto Carbonell

2.        Analysis of the Uridylation of Both ARGONAUTE–Bound MiRNAs and 5’ Cleavage Products of their Target RNAs in Plants

Guodong Ren, Xiaoyan Wang, and Bin Yu

3.        In Vitro Formation of Plant RNA-Induced Silencing Complexes Using an Extract of Evacuolated Tobacco Protoplasts

Taichiro Iki, Masayuki Ishikawa, and Manabu Yoshikawa

4.        In vitro Analysis of ARGONAUTE–Mediated Target Cleavage and Translational Repression in Plants

Yukihide Tomari and Hiro–oki Iwakawa

5.        Establishment of an In Vivo ARGONAUTE Reporter System in Plants

Károly Fátyol and József Burgyán

6.        Immunoprecipitation and High–throughput Sequencing of ARGONAUTE–Bound RNAs from Plants

Alberto Carbonell<

7.        Identification of ARGONAUTE/Small RNA Cleavage Sites by Degradome Sequencing

8.        Dissecting the Sub–Nuclear Localization Patterns of ARGONAUTE Proteins and Other Components of the RNA–Directed DNA Methylation Pathway in Plants

Cheng–Guo Duan and Jian–Kang Zhu

9.        Detection of ARGONAUTE 1 Association with Polysomes in Arabidopsis thaliana

Cécile Lecampion, Elodie Lanet, and Christophe Robaglia

10.    Functional Analysis of Arabidopsis ARGONAUTEs in Meiosis and DNA Repair

Marina Martinez–Garciaand Mónica Pradillo

Tomohito Yamasaki

Meizhen Wang, Yuxing Deng, Fenjuan Shao, Miaomiao Liu, Yongqi Pang, Caili Li, and Shanfa Lu

13.    Cloning and Characterization of ARGONAUTE Genes in Tomato

Zhiqiang Xian, Fang Yan, and Zhengguo Li

Ariel H. Tomassi, Delfina Gagliardi, Damian A. Cambiagno, and Pablo A. Manavella

15.    Non–Radioactive Plant Small RNA Detection Using Biotin–Labeled Probes

Jun Hu and Yingguo Zhu

16.    Computational Analysis of Genome–Wide ARGONAUTE–Dependent DNA Methylation in Plants

Kai Tang, Cheng–Guo Duan, Huiming Zhang and Jian–Kang Zhu

17.    Structural and Functional Characterization of Plant ARGONAUTE MID Domains

Filipp Frank and Bhushan Nagar

18.    Identification and Analysis of WG/GW ARGONAUTE–Binding Domains

Andrzej Zielezinski and Wojciech M. Karlowski

19.    Identification and Evolutionary Characterization of ARGONAUTE-Binding Platforms

Joshua T. Trujillo and Rebecca A. Mosher

20.    Phylogenetic and Evolutionary Analysis of Plant ARGONAUTES

Ravi K. Singh and Shree P. Pandey

Includes cutting-edge methods and protocols.

Provides step-by-step essential for reproducible results.

Contains key notes and implementation advice from the expert

Includes supplementary material: sn.pub/extras

Includes supplementary material: sn.pub/extras