Plant Endosomes (2^nd Ed., 2nd ed. 2020)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2177

This second edition details techniques for the study of cargo trafficking through endosomes. New and updated chapters guide readers through methods and protocols on the structural aspects of plant endosomes, combined biochemical, omics, imaging approaches to study the dynamics and contents of endosomal compartments. Additional chapters are dedicated to the analysis of lipids on endosomes and the identification and analysis of lipid binding proteins and lipid-binding domains relevant for the study of plant endosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Endosomes: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

1. Analysis of Endocytosis and Intracellular Trafficking of Boric Acid/Borate Transport Proteins in Arabidopsis

Akira Yoshinari and Junpei Takano

2. Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles

Mengqian Luo, Ying Zhu, Zhiqi Liu, and Liwen Jiang

3. Analysis of Endoplasmic Reticulum-Endosomes Association Using Live-Cell Imaging In Plant Cells

Giovanni Stefano and Federica Brandizzi

4. Degradation of Abscisic Acid Receptors through the Endosomal Pathway

Borja Belda-Palazón and Pedro L. Rodriguez

5. Biochemical and Imaging Analysis of ALIX Function in the Endosomal Trafficking of Arabidopsis Protein Cargoes

Marta García-León and Vicente Rubio

6. Correlative Light and Electron Microscopy Imaging of the Plant trans-Golgi Network

Pengfei Wang and Byung-Ho Kang

7. Imaging Plant Cells by High-pressure Freezing and Serial Block-Face Scanning Electron Microscopy

Kirk Czymmek, Abhilash Sawant,Kaija Goodman, Janice Pennington, Pal Pedersen, Mrinalini Hoon, and Marisa S. Otegui

8. Functional Analysis of Plant FYVE Domain Proteins in Endosomal Trafficking

10. Subcellular Localization of PI3P in Arabidopsis

Han Nim Lee, Hyera Jung, and Taijoon Chung

12. Cell-free Protein Translation System For Expression Of Lipid-binding Proteins Tagged with Small Epitopes and their use in Protein-lipid Overlay Assays

13. Isolation and Glycomic Analysis of Trans-Golgi Network Vesicles in Plants

Guangxi Ren, Michel Ruiz Rosquete, Angelo G. Peralta, Sivakumar Pattathil,Michael G. Hahn, Thomas Wilkop,and Georgia Drakakaki

Karin Vogel and Erika Isono

15. Purification and Interaction Analysis of a Plant-specific RAB5 Effector by in vitro pull-down Assay 

Emi Ito, Seung-won Choi, and Takashi Ueda

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts