Plant Stem Cells, 1st ed. 2020
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2094

This volume looks at the latest technologies and methods used by researchers to study mechanisms that control the aspects of plant stem cell signaling events. The topics discussed in this book cover shoot apical meristem inoculation assay; plant associated microbes that alter root growth by modulating root apical meristem; visualizing auxin and cytokinins signaling activity; 3D analysis of mitosis distribution pattern in the plant root tip with iRoCS toolbox; and micropropagation of rosaceous species SAM grown in temperate climate. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and authoritative, Plant Stem Cells: Methods and Protocols is a useful resource for all researchers interested in learning more about this developing field.

Preface…

Table of Contents…

Contributing Author… 

1. Regulatory Role of Phytohormones in Maintaining Stem Cells and Boundaries of Stem Cell Niches

Aqib Syed, Anwar Hussain, Waheed Murad, and Badshah Islam   

2. Bacterial Shoot Apical Meristem Inoculation Assay 

Muhammad Naseem, Gökhan Gun, Ozge Osmanoglu, Fatima A. AlRemeithi, Jibran Iqbal, Ihsan Ullah, and Thomas Dandekar

3. Germline-Transmitted Genome Editing Methodology in Arabidopsis thaliana using TAL-Effectors Nucleases

Joachim Forner

4. Method to Study Gene Expression Patterns during de novo Root Regeneration from Arabidopsis Leaf Explants

Jie Yu, Ning Zhai, Lin Xu, and Wu Liu

5. Labeling and Sorting of Arabidopsis SAM Cell Populations to Capture their Transcriptome Profile 

Monika Mahajan and Ram Kishor Yadav

6. Plant Associated Microbes Alter Root Growth by Modulating Root Apical Meristem

Anwar Hussain, Husna Ullah, Ihsan Ullah, and Muhammad Naseem 

7. Live Imaging of Arabidopsis Axillary Meristems

Bihai Shi, Hongli Wang, and Yuling Jiao

8. Molecular Modeling of the Interaction between Stem Cells Peptide and Immune Receptor in Arabidopsis

Muhammad Naseem, Mugdha Srivastava, Ozge Osmanoglu, Jibran Iqbal, Fares M. Howari, Fatima A. AlRemeithi, and Thomas Dandekar

9. Methods to Visualize Auxin and Cytokinins Signaling Activity in the Shoot Apical Meristem

Géraldine Brunoud, Carlos S. Galvan-Ampudia, and Teva Vernoux

10. Analysis of Cell Division Frequency in the Root Apical Meristem of Lycophytes, Non-Seed Vascular Plants, using EdU Labeling

Rieko Fujinami

11. Osmotic Treatment for Quantifying Cell Wall Elasticity in the Sepal of Arabidopsis thaliana

12. Mapping a Transcriptome Guided Arabidopsis SAM Interactome

Muhammad Naseem, Ozge Osmanoglu, Jibran Iqbal, Fares M. Howari, Fatima A. AlRemeithi, Martin Kaltdorf, and Thomas Dandekar

13. 3D Analysis of Mitosis Distribution Pattern in the Plant Root Tip with iRoCS Toolbox

Viktoriya V. Lavrekha, Taras Pasternak, Klaus Palme, and Victoria V. Mironova

14. Micropropagation of Rosaceous Species SAM Grown in Temperate Climate

Jiri Sedlak and Frantisek Paprstein

15. A New Perspective on Cryotherapy: Pathogen Elimination using Plant Shoot Apical Meristem via Cryogenic Techniques

Ergun Kaya, Selin Galtali, Sevinc Gultag, and Onur Celik