Plant Stress Tolerance (2^nd Ed., 2nd ed. 2017)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1631

This second edition provides up-to-date and new chapters covering the most important widely-used techniques in plant stress tolerance, including cutting-edge strategies, in a manner that ensures effective results. Plant Stress Tolerance: Methods and Protocols, Second Edition guides readers through reviews on epigenetics, long noncoding RNAs, and the influence of microbiome on drought tolerance, the volume then continues with methods describing the identification of stress-responsive genes, proteins, posttranslational redox modifications, DNA methylation, histone modifications, measurement of glutathione, lipids, raffinose family oligosaccharides, polyamines, as well as quantification of abscisic acid, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Stress Tolerance: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Part I: Review Chapters

1. Epigenetics and RNA Processing: Connections to Drought, Salt and ABA?

Min May Wong, Geeng Loo Chong and Paul Verslues

2. The Fundamental Role Of Reactive Oxygen Species In Stress Response

Michael Liebthal and Karl-Josef Dietz

3. Role of Long Non-coding RNAs on Plant Stress Tolerance

Yuepeng Song and Deqiang Zhang

4. Towards a Resilient, Functional Microbiome: Drought Tolerance-Alleviating Microbes for Sustainable Agriculture

Part II: Methodology Chapters

5. Mining and Quantifying In Vivo Molecular Interactions in Abiotic Stress Acclimation

Thorsten Seidel and Derya Kirasi

6. Generation of a Stress-inducible Luminescent Arabidopsis and Its Use in Genetic Screening for Stress-responsive Gene Deregulation Mutants

Si-in Yu and Byeong-ha Lee

Ibtisam Al Harrasi, Rashid Al-Yahyai and Mahmoud W Yaish 

8. ChIP-seq Analysis for Identifying Genome-wide Histone Modifications Associated With Stress-responsive Genes in Plants  

Guosheng Li, Guru Jagadeeswaran, Andrew Mort, and Ramanjulu Sunkar

9. Isolation of Polysomal RNA for Analyzing Stress-Responsive Genes Regulated at the Translational Level in Plants

Yong-Fang Li, Ramamurthy Mahalingam, and Ramanjulu Sunkar

10. Global Proteomic Profiling and Identification of Stress-Responsive Proteins using Two-dimensional Gel Electrophoresis

Pragya Barua, Deepak Gayen, Nilesh Vikram Lande, Subhra Chakraborty, and Niranjan Chakraborty

11. Phosphoproteomics Analysis for Probing Plant Stress Tolerance

Christof Rampitsch

12. Probing Posttranslational Redox Modifications

Patrick Treffon, Michael Liebthal, Wilena Telman, and Karl-Josef Dietz

13. Zymographic Method for Distinguishing Different Classes of Superoxide Dismutases in Plants

Ashwini R. Jamdhade, Ramanjulu Sunkar, and Vandana K. Hivrale

14. Determination of Enzymes Associated with Sulfite Toxicity in Plants: Kinetic Assays For SO, APR, SiR and in-gel SiR Activity  

Galina Brychkova, Assylay Kurmanbayeva, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, and Moshe Sagi

15. Determination of Total Sulfur, Sulfate, Sulfite, Thiosulfate, and Sulfolipids in Plants

Assylay Kurmanbayeva, Galina Brychkova, Aizat Bekturova, Inna Khozin, Dominic Standing, Dmitry Yarmolinsky, and Moshe Sagi

16. Determining Glutathione Levels in Plans

Smita Sahoo, Jay Prakash Awasthi, Ramanjulu Sunkar, and Sanjib Kumar Panda

17. Porous Graphitic Carbon Liquid Chromatography-Mass Spectrometry Analysis of Drought Stress-responsive Raffinose Family Oligosaccharides in Plant Tissues

Tiago F. Jorge, Maria H. Florêncio^, and Carla António

18. Profiling Abscisic Acid Induced Changes in Fatty Acid Composition in Mosses

Suhas Shinde, Shivakumar Devaiah and Aruna Kilaru

Xiaoqing Gong and Jihong Liu

20. Determination of Polyamines by Dansylation, Benzoylation, and Capillary Electrophoresis

G. Veeranagamallaiah and Chinta Sudhakar

21. Rapid Quantification of Abscisic Acid by GC-MS/MS for studies of Abiotic Stress Response

Paul E. Verslues

22. Silencing of Stress-regulated miRNAs in Plants by Short Tandem Target Mimic (STTM) Approach

23. Rhizosphere Sampling Protocols for Microbiome (16S/18S/ITS rRNA) Library Preparation and Enrichment for the Isolation of Drought Tolerance-Promoting Microbes 

Venkatachalam Lakshmanan, Prasun Ray, and Kelly D. Craven 

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts?

Includes supplementary material: sn.pub/extras

Includes supplementary material: sn.pub/extras