Plant Vacuolar Trafficking, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1789

This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a  highly valuable resource for researchers interested in learning more about this field

1. Green-fluorescent Seed, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds

Tomoo Shimada,  Kentaro Fuji, Takuji Ichino; Ooi-Kock The, Yasuko KoumotoIkuko, and Hara-Nishimura

2. Detection of CRISPR/Cas9 Induced Genomic Fragment Deletions in Barley and Generation of Homozygous Edited Lines via Embryogenic Pollen Culture

Eszter Kapusi and Eva Stöger

Bruno Peixoto, Susana Pereira, Cláudia Pereira, and José Pissarra

4. Vacuole-targeted Proteins: Ins and Outs of Subcellular Localization Studies

Inês Carqueijeiro, Liuda J. Sépulveda, Angela Mosquera, Richard Payne, Cyrielle Corbin, Nicolas Papon, Thomas Dugé de Bernonville, Sébastien Besseau,  Arnaud Lanoue, Gaëlle Glévarec, Marc Clastre, Benoit St-Pierre; Lucia Atehortùa, Nathalie Giglioli-Guivarc’h, Sarah E. O’Connor, Audrey Oudin, and Vincent Courdavault

5.         Plant Cell Vacuoles: Staining and Fluorescent Probes

Giovanni Stefano, Luciana Renna, and Federica Brandizzi

Carolina Gabriela Ocampo and Silvana Petruccelli

7. Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus

Inês Carqueijeiro, Henrique Noronha, Sara Bettencourt, Joana G. Guedes,  Patrícia Duarte, Hernâni Gerós, and Mariana Sottomayor

8. Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells

Jorge Rodrigues, Cátia Pereira, Natacha Fontes, Hernâni Gerós, and Manuela Côrte-Real

9. Live cell Imaging and Confocal Microscopy

Luciana Renna, Giovanni Stefano, and Federica Brandizzi

10. Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM)

Alexandra Chanoca,  Brian Burkel, Erich Grotewold, Kevin W. Eliceiri, and Marisa S. Otegui

11. The Use of Drugs in the Study of Vacuole Morphology and Trafficking to the Vacuole in Arabidopsis thaliana

Ricardo Tejos, Claudio Osorio-Navarro, and Lorena Norambuena

Junpei Takagi and Tomohiro Uemura

13. Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking

Lei Huang and Chunhua Zhang

Alessandro Occhialini

Kiersun Jones and Chang Hyun Khang

16. Characterization Of Plant Glycoproteins: Analysis of Plant Glycopeptide Mass Spectrometry Data with plantGlycoMS, a Package in the R Statistical Computing Environment

Margaret R. Baker, Travers Ching, David L. Tabb, and Qing X. Li

Includes cutting-edge methods and protocols

Provides step-by-step detail essential from reproducible results

Contains key notes and implementation advice from the experts