Plasmodesmata, Softcover reprint of the original 1st ed. 2015
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1217

Plasmodesmata: Methods and Protocols provides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their function during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular trafficking and movement through PD, the book continues with detailed experimental protocols for imaging PD, the isolation and structural analysis of PD, the analysis of PD conductivity and regulation, and for studying cellular mechanisms by which macromolecules are targeted to PD and transported through the channel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to use, Plasmodesmata: Methods and Protocols delivers detailed information about specific practical procedures in order to enable their reproduction and application in PD-related research in laboratories around the world.

Plasmodesmata: Channels for Intercellular Signaling During Plant Growth and Development.- Plasmodesmata: Channels for Viruses on the Move.- Imaging Plasmodesmata with High Resolution Scanning Electron Microscopy.- Preparative Methods for Imaging Plasmodesmata at Super-Resolution.- Isolation of Plasmodesmata from Arabidopsis Suspension Culture Cells.- Immunofluorescence Detection of Callose Deposition around Plasmodesmata Sites.- Imaging Callose at Plasmodesmata Using Aniline Blue: Quantitative Confocal Microscopy.- Localization of Fluorescently-Tagged Protein to Plasmodesmata by Correlative Light and Electron Microscopy.- Quantification of Plant Cell Coupling with Live-Cell Microscopy.- Drop-ANd-See: A Simple, Real-Time, and Non-Invasive Technique for Assaying Plasmodesmal Permeability.- Mapping Symplasmic Fields at the Shoot Apical Meristem Using Iontophoresis and Membrane Potential Measurements.- Analysis of the Conductivity of Plasmodesmata by Microinjection.- Investigating Plasmodesmata Genetics with Virus-Induced Gene Silencing and an Agrobacterium-Mediated GFP Movement Assay.- Probing Plasmodesmata Function with Biochemical Inhibitors.- GAL4 Transactivation-Based Assay for the Detection of Selective Intercellular Protein Movement.- Techniques for Assessing the Effects of Pharmacological Inhibitors on Intercellular Protein Movement.- Probing Protein Targeting to Plasmodesmata Using Fluorescence Recovery after Photo-Bleaching.- The Tracking of Intercellular Small RNA Movement.- Analysis of the Role of Myosins in Targeting Proteins to Plasmodesmata.- Pumilio-Based RNA In Vivo Imaging.- In Vivo RNA Labeling Using MS2.