Rice Grain Quality, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1892

This volume explores the latest advances in high-throughput phenotyping to screen milling quality, cooking and nutritional quality in breeding material, and simplifying grain quality screening in germplasm. The chapters in this book cover topics such as seed development stages; panicle architectural traits to understand yield components; starch structure and protein properties; and genome editing technologies to reveal grain quality and nutritional traits. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Rice Grain Quality: Methods and Protocols is a valuable resource for all researchers interested in expanding their knowledge in this evolving field.

Table of Contents…

Contributors…

1. Improving Head Rice Yield and Milling Quality: State of the Art and Future Prospects 

Vito M. Butardo, Jr. and Nese Sreenivasulu

2. Improving Rice Grain Quality: State of the Art and Future Prospects

Vito M. Butardo, Jr., Nese Sreenivasulu, and Bienvenido O. Juliano

3. Morphology of Rice Seed Development and Its Influence on Grain Quality

Paul A. Counce and Karen A.K. Moldenhauer

4. Novel Imaging Techniques to Analyze Panicle Architecture

Erstelle Pasion, Roinand Aguila, Nese Sreenivasulu, and Roslen Anacleto

5. Measuring Head Rice Recovery in Rice

Jennine Rose Lapis, Rosa Paula Cuevas, Nese Sreenivasulu, and Lilia Molina

6. Measurement of Rice Grain Dimensions and Chalkiness, and Rice Grain Elongation

Using Image Analysis

Marnol Santos, Rosa Paula Cuevas, Nese Sreenivasulu, and Lilia Molina

7. Method Development of Near-Infrared Spectroscopy Approaches for Non-Destructive

and Rapid Estimation of Total Protein in Brown Rice Flour

Rosario Jimenez, Lilia Molina, Iman Zarei, Jennine Rose Lapis, Ruben Chavez, Rosa Paula O. Cuevas, and Nese Sreenivasulu

8. Multi-Dimensional Cooking Quality Classification Using Routine Quality Evaluation Methods

Lilia Molina, Rosario Jimenez, Nese Sreenivasulu, and Rosa Paula Cuevas

9. Characterization of Mechanical Texture Attributes of Cooked Milled Rice by Texture Profile Analyses and Unraveling Viscoelasticity Properties through Rheometry

Rosa Paula Cuevas, Pawan S. Takhar, and Nese Sreenivasulu

10. Characterizing Starch Molecular Structure of Rice

Cheng Li, Hongyan Li, and Robert G. Gilbert

in Grains Using Gas Chromatography Mass Spectrometry

Cindy Llorente, Rosarion Jimenez, Jackie, Yariv Brotman, Alisdair R. Fernie, and Nese Sreenivasulu

12. Re-Sequencing Resources to Improve Starch and Grain Quality in Rice

Gopala Krishnan S, Ardashir K. Masouleh, Agnelo Furtado, Daniel L. E. Waters, and 

Robert J. Henry

13. Quantifying Grain Digestibility of Starch Fractions in Milled Rice

Crisline Mae Alhambra, Sushil Dhital, Nese Sreenivasulu, and Vito M. Butardo, Jr.

14. Determination of Macronutrient and Micronutrient Content in Rice Grains Using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES)

Lilia Molina, Jennine Rose Lapis, Nese Sreenivasulu, and Rosa Paula Cuevas

15. Determination of Cadmium Concentration in Milled and Brown Rice Grains Using Graphite Furnace Atomic Absorption Spectrometry

Lilia Molina, Jennine Rose Lapis, Nese Sreenivasulu, and Rosa Paula Cuevas

16. Analysis of Developing Rice Grain Transcriptome Using the Agilent Microarray Platform

Mandy Püffeld, Christiane Seiler, Markus Kuhlmann, Nese Sreenivasulu, and Vito M. Butardo, Jr.

17. Quantification of DNA Methylation as Biomarker for Grain Quality

Christiane Seiler and Markus Kuhlmann

18. CRISPR-Cas9 Mediated Genome Editing of Rice towards Better Grain Quality

Anindya Bandyopadhyay, Xiaojia Yin, Akshaya Biswal, Robert Coe, and William Paul Quick