Angiogenesis Assays: A Critical Appraisal of Current Techniques
A Critical Appraisal of Current Techniques

This book describes in detail the in vitro and in vivo assays currently being used to study angiogenesis, including the provision of protocols. The chapters are written by leading scientists who use these techniques in their angiogenesis research programmes and who deliver here a critical appraisal of their strengths and weaknesses.

Endothelial cell biology. Endothelial cell proliferation assays. Tubule formation assays. Definitions. Assaying endothelial-mural cell interactions. Assays for membrane and intracellular signalling events. Implantation of sponges and polymers. Angiogenesis assays in the chick. Corneal angiogenesis assay. Dorsal air sac model. Chamber assays. Tumour models: analysis of angiogenesis in vivo. Fluorescent proteins to image angiogenesis. Visualizing the developing vasculature. Biological and clinical implications of recruitment of stem cells into angiogenesis. Tumour micro-environmental factors. Possible mechanisms of acquired resistance to anti-angiogenic drugs. In vitro assays. Index.

Dr CA Staton. Lecturer in Angiogenesis Research, Academic Unit of Surgical Oncology, University of Sheffield Medical School.

Professor Claire Lewis. Professor of Molecular & Cellular Pathology, Academic Unit of Pathology, University of Sheffield Medical School.

Professor Roy Bicknell. CRUK Molecular Angiogenesis Labs, Institute of Molecular Medicine, University of Oxford.