Description
Bacterial Polysaccharides, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1954
Coordinator: Brockhausen Inka
Language: EnglishSubjects for Bacterial Polysaccharides:
312 p. · 17.8x25.4 cm · Hardback
Description
/li>Contents
/li>Comment
/li>
This book provides a selection of recently developed methods and protocols in bacterial glycomics to aid in bettering our understanding of the structures and functions of bacterial polysaccharides, their attachments to proteins and lipids, their role in biofilm formation, as well as their biosynthesis. With the emerging bacterial resistance to commonly used antibiotics world-wide, these techniques to study the outer polysaccharides of bacteria, with their functions in bacterial adhesion, colonization, growth, establishment of biofilms, and control virulence and pathogenicity, are increasingly important. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bacterial Polysaccharides: Methods and Protocols aims to support researchers contributing to future approaches that will fill our knowledge gaps and define anti-bacterial targets.
2. Biosynthesis of complete O antigen repeating units in pathogenic bacteria
3. S-layer biosynthesis4. Synthesis of mammalian glycoconjugates in Neisseria
<5. Polymerizing Mannosyltransferases6. Chain length and termination control
7. O antigen Polymerases
8. Polysialyltransferases in bacteria
9. Polymerizing Sialyl-hexose transferase
10. Kdo-transferases and capsule biosynthesis
10. Production of legionaminic acids by sialyltransferases
11. Outer core L-rhamnosyltransferases that control O antigen attachment
12. Production of human blood group epitopes in E.coli
13. Chemoenzymatic synthesis of undecaprenyl phosphate analogs
14. Inhibitor of undecaprenyl diphosphate synthase
15. Chemical synthesis of TDP-Rha16. Synthesis of TDP-L-Rha analogs
17. Simplified synthesis of nucleotide sugars
18. Inhibition of bacterial glycosyltransferases
19. Glycosyltransferase protein structure determination
20. Crystal Structure of MurNAc-α1-P Uridylyltransferase MurU
21. O-acetylation in peptidoglycans
22. Assays of nucleotide sugar-dependent glycosyltransferases
23. Mechanism of Staphylococcus aureus cell wall teichoic Acid β-glycosyltransferase involved in Methicillin resistance.
24. Mycobacterial arabinosyl-transferase activity
25. Immunogenicity of bacterial polysaccharides26. Receptor binding of bacteriophages
27. Bacterial N-glycosylation
28. Protein O-glycosylation in bacteria
29. Role of polysaccharide in biofilm formation
30. O antigens and biofilms
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts