Biophysics of Membrane Proteins, 1st ed. 2020
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2168

This volume provides recent advances in the field of biophysics of membrane proteins. Chapters are divided into several parts: detailing biochemistry and functional analysis, experimental and theoretical structural determinations, membrane protein dynamics, and conformation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Biophysics of Membrane Proteins: Methods and Protocols aims to provide comprehensive protocols with notes to help further the understanding of key membrane protein structure and function for students, academics, and industrial researchers.

Part I: Biochemistry and Functional Analysis

1. Membrane Protein Production and Purification from Escherichia coli and Sf9 Insect Cells

Yixin Liu, Ana Pavić, Joshua T. Farley, Carine de Marcos Lousa, Adrian Goldman, and Vincent L. G. Postis

2. Quantifying the Interaction of Phosphite with ABC Transporters: MicroScale Thermophoresis and a Novel His-tag Labeling Approach

Tanja Bartoschik, Amit Gupta, Beate Kern, Andrew Hitchcock, Nathan B.P. Adams, and Nuska Tschammer

Dhenesh Puvanendran, Hager Souabni, Dimitri Salvador,  Olivier Lambert, Quentin Cece, and Martin Picard

4. Functional Characterization of SLC transporters using Solid-Supported-Membranes

Andre Bazzone and Maria Barthmes

5. Thermostability Assays Provide a Generic and Versatile Tool for Studying the Functional and Structural Properties of Membrane Proteins in Detergents

Steven P.D. Harborne, Martin S. King, and Edmund R.S. Kunji

6. Direct monitoring of GPCR reconstitution and ligand binding activity by Plasmon Waveguide Resonance

Isabel D. Alves

Part II: Experimental and Theoretical Structural Determination

7. Examining Membrane Proteins by Neutron Scattering

Christine Ebel, Cécile Breyton, and Anne Martel

8. Solution X-ray Scattering for Membrane Protein

Maciej Baranowski and Javier Pérez

9. Interpreting SAXS/WAXS Data with Explicit-Solvent Simulations: A Practical Guide

Markus Rainer Hermann and Jochen Sebastian Hub

10. Determining the Free Energies of Outer Membrane Proteins in Lipid Bilayers

Gerard H M Huysmans, Dagan C Marx, Sheena E Radford,and Karen G Fleming

11. Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry

Dietmar Hammerschmid, Jeroen F. van Dyck, Frank Sobott, and Antonio N. Calabrese

12. Determination of the Molecular Mass of Membrane Proteins Using Size Exclusion Chromatography with Multi-angle Laser Light Scattering (SEC-MALLS)

Maren Thomsen

Part III: Membrane Protein Dynamics and Conformations

Tomas Fessl, Matthew A Watson, Daniel Watkins, Marek Scholz, Joel Crossley,  Tara Sabir, Sheena E. Radford, Ian Collinson, and RomanTuma

14. Short-Range Distances Measurement by Transition Metal Ion FRET

Jonas S. Mortensen and Claus J. Loland

15. PELDOR/DEER – An Electron Paramagnetic Resonance Method to Study Membrane Proteins in Lipid Bilayers

Martin F. Peter, Kiran Bountra, Konstantinos Beis, and Gregor Hagelueken

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts