Capillary Electrophoresis of Proteins and Peptides, 1st ed. 2016
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1466

Language: English
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Capillary Electrophoresis of Proteins and Peptides
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This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.

1. Chemical and Instrumental Approaches for Capillary Electrophoresis (CE)-Fluorescence Analysis of Proteins

 Isabelle Le Potier, Audrey Boutonnet, Vincent Ecochard, and François Couderc

 

2. Discrimination of Glycoproteins from Unglycosylated Proteins in Capillary Electrophoresis: Two-Color LIF Detection Coupled with Post-Column Derivatization

  Takashi Kaneta

 

3. Capillary Zone Electrophoresis-Mass Spectrometry of Intact Proteins

  Elena Domínguez-Vega, Rob Haselberg, and Govert W. Somsen

 

4. Screening of Small Intact Proteins by Capillary Electrophoresis Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)

  Sabine Neuberger, Angelina Rafai, and Christian Neusüß

 

5. Online Capillary IsoElectric Focusing-ElectroSpray Ionization Mass Spectrometry (CIEF-ESI MS) in Glycerol-Water Media for the Separation and Characterization of Hydrophilic and Hydrophobic Proteins

  Meriem Mokaddem, Fanny d’Orlyé, and Anne Varenne

 

6. On-Line Solid-Phase Extraction Capillary Electrophoresis Mass Spectrometry for Preconcentration and Clean-Up of Peptides and Proteins

 Fernando Benavente, Silvia Medina-Casanellas, Estela Giménez, and Victoria Sanz-Nebot

 

7. Affinity Monolith-Integrated Microchips for Protein Purification and Concentration

  Changlu Gao, Xiuhua Sun, Huaixin Wang, Wei Qiao, and Bo Hu

 

8. Analysis of Somatropin by Double Injection Capillary Zone Electrophoresis in Polybrene/Chondroitin Sulfate A Double Coated Capillaries

  Ahmad Amini

 

9. Poly(N,N-Dimethylacrylamide)-Based Coatings to Modulate Electroosmotic Flow and Capillary Surface Properties for Protein Analysis

  Laura Sola, Marina Cretich, and Marcella Chiari

 

10. Measurement of Inflammatory Chemokines in Micro-Dissected Tissue Biopsy Samples by Chip-Based Immunoaffinity Capillary Electrophoresis

  Terry M. Phillips, Edward Wellner, Shane McMohan, and Heather Kalish

 

11. Separation of Recombinant Therapeutic Proteins Using Capillary Gel Electrophoresis and Capillary Isoelectric Focusing

            Caitlyn A.G. De Jong, Jessica Risley, Shuai Sherry Zhao, and David D.Y. Chen

 

12. Characterization of Chemical and Physical Modifications of Human Serum Albumin by Capillary Zone Electrophoresis

 Anne-Lise Marie, Nguyet Thuy Tran, and Myriam Taverna

 

13. Capillary Electrophoresis Method for the Assessment of Erythropoiesis Stimulating Agents in Final Formulations

  Michel Girard, Anita Kane, and Sylvie Boucher

 

14. Quality Control of Therapeutic Monoclonal Antibodies at the Hospital After Their Compounding and Before Their Administration to Patients

 Emmanuel Jaccoulet, Claire Smadja, and Myriam Taverna

 

15. Capillary Electrophoresis Ultraviolet Mass Spectrometry (CE-UV-MS) for the Simultaneous Determination and Quantitation of Insulin Formulations

 Julie Schappler and Serge Rudaz

 

16. Applications of an Automated and Quantitative CE-Based Size and Charge Western Blot for Therapeutic Proteins and Vaccines

 Richard R. Rustandi, Melissa Hamm, Catherine Lancaster, and John W. Loughney

 

17. Affinity Capillary Electrophoresis Applied to Investigation of Valinomycin Complexes with Ammonium and Alkali Metal Ions

  Sille Štěpánová and Václav Kašička

Includes cutting-edge methods and protocols for the study of CE of proteins and peptides

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras