Cardiovascular Proteomics, 2007
Methods and Protocols
Methods in Molecular Biology Series, Vol. 357

Over the past few years, the power and potential of proteomics has become widely recognized. The use of proteomics for the study of complex diseases is increasing and is particularly applicable to cardiovascular disease, the leading cause of death in developed countries. The ability to investigate the complete proteome provides a critical tool toward elucidating the complex and multif- torial basis of cardiovascular biology, especially disease processes. Proteomics involves the integration of a number of technologies with the aim of analyzing all the proteins expressed by a biological system in response to various stimuli under different pathophysiological conditions. The proteomic approach offers the ability to evaluate simultaneously the changes in protein expression and cell signaling pathways in response to such conditions as atherosclerosis, c- diac hypertrophy, stroke, or heart failure. Cardiovascular Proteomics: Methods and Protocols covers many of the above aspects of the proteomic approach in the cardiovascular field. This v- ume takes the reader through the complete process of proteomic analysis, from the obtention of specific heart proteins (troponin I) to the new techniques of identifying risk biomarkers of atherome plaque rupture, analyzing the secretome of explanted endarterectomies cultured in vitro, or the application of phage display techniques to decipher the molecular diversity of blood vessels.

Analysis of the Heart Proteome.- Two-Dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics.- Analyzing the Cardiac Muscle Proteome by Liquid Chromatography-Mass Spectrometry-Based Expression Proteomics.- Two-Dimensional Differential Gel Electrophoresis of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion.- Analysis of Antihypertensive Drugs in the Heart of Animal Models.- A Solubility Optimization Protocol for Two-Dimensional Gel Electrophoresis of Cardiac Tissue.- A Method for the Effective Depletion of Albumin From Cellular Extracts.- Heart Subproteomes.- Subcellular Fractionation.- Subfractionation of Heart Tissue.- Optimization of Cardiac Troponin I Pull-Down by Idm Affinity Beads and SELDI-TOF.- Proteomic Analysis of the Subunit Composition of Complex I (NADH:Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria.- Identification of Targets of Phosphorylation in Heart Mitochondria.- Proteomics of Human Atheroma Plaques.- Characterization of the Human Atheroma Plaque Secretome by Proteomic Analysis.- Characterization of HSP27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome.- Western Array Analysis of Human Atherosclerotic Plaques.- The Proteome of Cells of the Cardiovascular System.- The Proteome of Endothelial Cells.- Proteomic Study of Caveolae and Rafts Isolated From Human Endothelial Cells.- Proteomic Identification of S-Nitrosylated Proteins in Endothelial Cells.- The Proteome and Secretome of Human Arterial Smooth Muscle Cell.- Real-Time In Vivo Proteomic Identification of Novel Kinase Substrates in Smooth Muscle.- Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain.- Isolation and Culture of Adult Mouse Cardiac Myocytes.- Proteomic Analysis of Foam Cells.- Isolation of the Platelet Releasate.- Enrichment of Phosphotyrosine Proteome of Human Platelets by Immunoprecipitation.- Characterization of Circulating Human Monocytes by Proteomic Analysis.- Cardiovascular Biomarkers in Plasma.- Cardiovascular Biomarkers Discovery by SELDI-TOF Mass Spectrometry.- Detection of Biomarkers of Stroke Using SELDI-TOF.- Depletion of High-Abundance Proteins in Plasma by Immunoaffinity Substraction for Two-Dimensional Difference Gel Electrophoresis Analysis.- A Rapid, Economical, and Reproducible Method for Human Serum Delipidation and Albumin and IgG Removal for Proteomic Analysis.- Bioinformatics: Emerging Technologies.- Mitoproteome.- Techniques to Decipher Molecular Diversity by Phage Display.

Includes supplementary material: sn.pub/extras