Cell-Based Microarrays, 1st ed. 2018
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1771

This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell microarrays, printing techniques for live-cell microarrays, live-cell microarray application, microfluidic live-cell arrays, and advanced materials suitable for live-cell microarrays. Some of the topics covered are artificial micro-niche array with spatially structured biochemical cues; inkjet printing for biomedical application; fabrication of multi-electrode arrays for neurobiology applications; rapid prototyping of thermoplastic microfluidic devices; and oxygen-generating photocrosslinkable hydrogel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cell-Based Microarrays: Methods and Protocols is a valuable resource for anyone interested in expanding their knowledge and research of cell-based microarrays.

Preface…

Table of Contents…

Contributing Authors…

Part I Introduction

1 Next Generation Live-Cell Microarray Technologies

Mario Rothbauer and Peter Ertl

Part II Biointerface Engineering & Live-Cell Microarrays

2 Cell-Based Microarrays Using Superhydrophobic Platforms Patterned with Wettable Regions  

Mariana B. Oliveira and João F. Mano

3 3D Plasma Nanotextured® Polymeric Surfaces for Protein or Antibody Arrays, Biomolecule, and Cell Patterning

Katerina Tsougeni, Kosmas Ellinas, George Koukouvinos, Panagiota S. Petrou, Angeliki Tserepi, Sotirios E. Kakabakos, and Evangelos Gogolides

4 Single Cell Microarrays Fabricated by Microscale Plasma-Initiated Protein Patterning (µPIPP)

Anita Reiser, Matthias L. Zorn, Alexandra Murschhauser, Joachim O. Rädler

5 Artificial Micro-Niche Array with Spatially Structured Biochemical Cues 

Xumei Gao, Celine Stoecklin, Yue Zhang, Zhe Weng, Richard de Mets, Gianluca Grenci, and Virgile Viasnoff

Part III Printing Techniques for Live-Cell Microarrays 

6 Three-Dimensional Cell-Based Microarrays: Printing Pluripotent Stem Cells into 3-D Microenvironments

Jorge F. Pascoal, Tiago G. Fernandes, Gregory J. Nierode, Maria M. Diogo, Jonathan S. Dordick, and Joaquim M. S. Cabral

7 Fabrication of Biomolecule Microarrays for Cell Immobilization using Automated Microcontact Printing

Julie Foncy, Aurore Estève, Amélie Degache, Camille Colin, Jean Christophe Cau, Laurent Malaquin, Christophe Vieu, and Emmanuelle Trévisiol

8 Hydrogel Printing Based on UV-Induced Projection for Cell-Based Microarray Fabrication

9 Inkjet Printing for Biomedical Applications

Christopher Chi Wai Tse and Patrick J. Smith

Part IV Live-Cell Microarray Application

10 High Throughput Screening of Senescence Markers in Hematopoietic Stem Cells Derived from Induced Pluripotent Stem Cells

Shyam Sushama Jose, Kamila Bendickova, and Jan Fric

11 Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems

Rebecca Jonczyk, Thomas Scheper, and Frank Stahl

12 Fabrication of Multi-Electrode Arrays for Neurobiology Applications

Mario Malerba, Hayder Amin, Gian N. Angotzi, Alessandro Maccione, and Luca Berdondini

Part V Microfluidic Live-Cell Arrays

13 Rapid Prototyping of Thermoplastic Microfluidic Devices 

Richard Novak, Carlos N. Pitti, and Donald E. Ingber

14 Fabrication of Biomolecule Microarrays using Rapid Photochemical Surface Patterning in Thiol–ene Based Microfluidic Devices

Alexander Jönsson and Josiane P. Lafleur

15 Fabrication and Operation of Microfluidic Hanging-Drop Networks

Patrick M. Misun, Axel K. Birchler, Moritz Lang, Andreas Hierlemann, and Olivier Frey

16 A Microfluidic Droplet Array System for Cell-Based Drug Combination Screening

Guan-Sheng Du, Jian-Zhang Pan, Shi-Ping Zhao, Ying Zhu, Jaap M.J. den Toonder, and Qun Fang

17 Establishment of Microfluidic Spheroid Cultures for Biomedical Applications

Karina Kwapiszewska

18 Capillary Pinning Assisted Patterning of Cell-Laden Hydrogel Microarrays in Microchips

Burcu Gumuscu and Jan C.T. Eijkel

Part VI Advanced Materials Suitable for Live-Cell Microarrays

19 Oxygen-Generating Photocrosslinkable Hydrogel

Neslihan Alemdar

20 A Self-Assembled Anti-Fouling Nano-Biointerface for the Generation of Spheroids 

Christoph Eilenberger, Mario Rothbauer, Peter Ertl, and Seta Küpcü

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts