Chloroplast Biotechnology (2nd Ed., 2nd ed. 2021)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2317

Coordinator: Maliga Pal

Language: English

137.14 €

In Print (Delivery period: 15 days).

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Chloroplast Biotechnology
Publication date:
369 p. · 17.8x25.4 cm · Paperback

232.09 €

In Print (Delivery period: 15 days).

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Chloroplast Biotechnology
Publication date:
369 p. · 17.8x25.4 cm · Hardback
This second edition volume expands on the previous edition with updated chapters in model systems and new crops. The book contains protocols for plastid engineering in leaves, tissue culture cells, and the shoot apex of plants, as well as for marker excision from the plastid genome and engineering Rubisco, the key enzyme of photosynthesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and practical, Chloroplast Biotechnology: Methods and Protocols, Second Edition is a valuable resource for researchers who wish to enter the field, and for practitioners looking for insights 
of applications in agriculture, industrial biotechnology and healthcare.  

Dedication…
Preface…
Table of Contents…
Contributing Authors…

Part I Background 

1. The Plastid Genomes of Flowering Plants: Essential Principles
Tracey A. Ruhlman and Robert K. Jansen

2. Plastid Gene Transcription: An Update on Promoters and RNA Polymerases 
Jennifer Ortelt and Gerhard Link 

3. Engineering Chloroplasts for High-Level Constitutive or Inducible Transgene Expression
Ralph Bock

4. Marker-Free Transplastomic Plants by Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences
Elisabeth A. Mudd, Panagiotis Madesis, Elena Martin Avila, and Anil Day

5. Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules
Maureen R. Hanson, Patricia L. Conklin, and Amirali Sattarzadeh 

Part II Nicotiana Tabacum: The Model Species of Chloroplast Biotechnology 

6. Transformation of the Plastid Genome in Tobacco: The Model System for Chloroplast Genome Engineering
Pal Maliga, Tarinee Tungsuchat Huang, and Kerry Ann Lutz 

7. Nicotiana tabacum: An Update on PEG-Mediated Plastid Transformation
Areli Herrera Díaz and Hans-Ulrich Koop 

8. Transformation of the Plastid Genome in Tobacco Suspension Cell Cultures
Jeffrey M. Staub 

9. Plastid Marker Gene Excision in the Tobacco Shoot Apex by Agrobacterium-Delivered Cre Recombinases 
Tarinee Tungsuchat-Huang and Pal Maliga 

10. Rubisco Engineering by Plastid Transformation and Protocols for Assessing Expression
Spencer M. Whitney and Robert E. Sharwood

Part III Crop Specific Plastid Transformation Protocols

11. Plastid Transformation in Tomato: A Vegetable Crop and Model Species
Stephanie Ruf and Ralph Bock

12. Stable Plastid Transformation of Petunia for Studies in Basic Science
Elena Martin Avila and Anil Day

13. Plastid Transformation in Potato: An Important Source of Nutrition and Industrial Materials
Vladimir T. Valkov, Daniela Gargano, Teodoro Cardi, and Nunzia Scotti 

14. Plastid Transformation of Poplar: A Model for Perennial Trees
Yuyong Wu, Ling Chang, Chunmei Jiang, Letian Xu, and Jiang Zhang 

15. Plastid Transformation in Lettuce (Lactuca sativa) by Biolistic DNA Delivery 
Tracey A. Ruhlman 

16. Plastid Transformation in Sugar Beet: An Important Industrial Crop
Francesca De Marchis and Michele Bellucci

Part IV Plastid Transformation in Algae

17. A Simple Technology for Generating Marker-Free Chloroplast Transformants of the Green Alga Chlamydomonas reinhardtii
Marco Larrea-Alvarez, Rosanna Young, and Saul Purton

18. Regulated Chloroplast Gene Expression in Chlamydomonas
Jean-David Rochaix, Raymond Surzycki, and Silvia Ramundo 

Part V Plastid Transformation in Bryophyte

19. Plastid Transformation in Physcomitrella patens: An Update
Mamoru Sugita

20. Plastid Transformation of Sporelings from the Liverwort Marchantia polymorpha L.
Shota Chiyoda, Katsuyuki T. Yamato, and Takayuki Kohchi

21. Rapid and Modular DNA Assembly for Transformation of Marchantia Chloroplasts
Eftychios Frangedakis, Kasey Markel, Susana Sauret-Gueto, and Jim Haseloff 

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts