Description
Chromatin Protocols (2nd Ed., 2nd ed. 2009)
Methods in Molecular Biology Series, Vol. 523
Coordinator: Chellappan Srikumar P.
Language: EnglishSubject for Chromatin Protocols:
Publication date: 08-2016
Support: Print on demand
Publication date: 03-2009
416 p. · 17.8x25.4 cm · Hardback
Description
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Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made in the last decade due to the development of novel techniques and the ability to sequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology? series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Chromatin Protocols, Second Edition is a valuable tool for scientists studying various aspects of chromatin function and an ideal guide to aid in the development of new techniques as well as new ideas in the field of chromatin biology.
Provides an easily accessible reference volume for analysis of chromatin structure and function incorporating the latest techniques
Includes detailed, step by step guidelines for the analysis of chromatin structure and its role in transcription, replication and other biological processes
Details elaborate protocols for the detection and characterization of histone modifications including acetylation, methylation and ubiquitination
Describes protocols for techniques ranging from immunohistochemical detection of chromatin modifications to genome-wide analysis of chromatin associated proteins
Acts as a comprehensive guide to the most up to date techniques dealing with all aspects of chromatin biology
Presents experimental details in an easy to follow modular format typical of the MiMB series
Includes supplementary material: sn.pub/extras