Description
Developmental Biology of the Sea Urchin and Other Marine Invertebrates (2nd Ed., 2nd ed. 2021)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2219
Coordinators: Carroll David J., Stricker Stephen A.
Language: EnglishSubjects for Developmental Biology of the Sea Urchin and Other Marine...:
Keywords
Sea stars; Embryos; Evolution; Novel model systems; Regenerative medicine; Oocytes
Publication date: 10-2021
309 p. · 17.8x25.4 cm · Paperback
Publication date: 10-2020
Support: Print on demand
Description
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1. Laboratory Culture and Mutagenesis of Amphioxus (Branchiostoma floridae)
Linda Z. Holland and Guang Li
2. Handling and Manipulation of Gametes and Embryos of the Annelidan Worm Pseudopotamilla occelata
Ryusaku Deguchi and Takeshi Nakano
3. The Starfish Asterina pectinifera: Collection and Maintenance of Adults and Rearing and Metamorphosis of Larvae
Naoyuki Murabe, Ei-ichi Okumura, Kazuyoshi Chiba, Enako Hosoda, Susumu Ikegami, and Takeo Kishimoto
4. Experimental Tools to Study Regeneration in the Sea Anemone Nematostella vectensis
Aldine R. Amiel and Eric Röttinger
5. Staining and Tracking Methods for Studying Sponge Cell Dynamics
Carole Borchiellini, Sandie M. Degnan, Emilie Le Goff, Caroline Rocher, Amélie Vernale, Stephen Baghdiguian, Nina Séjourné, Florent Marschal, André Le Bivic, Nelly Godefroy, Bernard M. Degnan, and Emmanuelle Renard
6. Microscopy Studies of Placozoans
Carolyn L. Smith, Tatiana D. Mayorova, Christine A. Winters, Thomas S. Reese, Sally P. Leys, and Andreas Heyland
7. Identification of SH2 Domain-Mediated Protein Interactions that Operate at Fertilization in the Sea Star Patiria miniata
Lauren Bates, Emily Wiseman, Jamie Kitson, and David J. Carroll
8. Marine Nemertean Worms for Immunoblotting Studies of Oocyte Aging
Stephen A. Stricker
9. Recovery of Sea Star Egg Cell Surface Proteins Released at Fertilization
Emily Wiseman, Lauren Bates, and David J. Carroll
10. Quantifying Cell Proliferation During Regeneration of Aquatic Worms
Eduardo E. Zattara and B. Duygu Özpolat
11. In Situ Hybridization Techniques in the Homoscleromorph Sponge Oscarella lobularis
Laura Fierro-Constain, Caroline Rocher, Florent Marschal, Quentin Schenkelaars, Nina Sejourné, Carole Borchiellini, and Emmanuelle Renard
12. Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond
Margherita Perillo, Periklis Paganos, Maxwell Spurrell, Maria I. Arnone, and Gary M. Wessel
13. Gene Editing in the Ascidian Phallusia mammillata and Tail Nerve Cord Formation
Alex McDougall, Celine Hebras, Isa Gomes, and Remi Dumollard
14. Transcriptomic Analysis in the Sea Anemone Nematostella vectensis
Jacob F. Warner and Eric Röttinger
15. RNA Interference on Regenerating Holothurian Gut Tissues
Miosotis Alicea-Delgado, Samir A. Bello-Melo, and José E. García-Arrarás
16. ATAC-Seq for Assaying Chromatin Accessibility Protocol Using Echinoderm Embryos
Marta Silvia Magri, Danila Voronov, Jovana Ranđelović, Claudia Cuomo, Jose Luis Gómez-Skarmeta, and Maria I. Arnone
17. Usage of the Sea Urchin Hemicentrotus pulcherrimus Database, HpBase
Sonoko Kinjo, Masato Kiyomoto, Takashi Yamamoto, Kazuho Ikeo, and Shunsuke Yaguchi
18. Functional Genomic Studies of Trichoplax adhaerens Neuronal Activity
Julia Gauberg, Adriano Senatore, and Andreas Heyland
19. A Bioinformatics Tutorial for Comparative Development Genomics in Diverse Meiofauna
Joseph L. Sevigny, Jon L. Norenburg, and Francesca Leasi
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
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