Description
Exocytosis and Endocytosis, 1st ed. 2021
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2233
Coordinators: Niedergang Florence, Vitale Nicolas, Gasman Stéphane
Language: EnglishSubjects for Exocytosis and Endocytosis:
Keywords
Amperometry; SNARE; Proteomics; Clathrin; flow cytometry; macropinocytosis
Publication date: 11-2021
329 p. · 17.8x25.4 cm · Paperback
Publication date: 11-2020
329 p. · 17.8x25.4 cm · Hardback
Description
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This volume provides basic and cutting-edge methods and protocols to study the major characteristics of eukaryotic cells. Chapters detail the different pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins involved in exocytosis, specific protocols for exocytosis in specialized cells such as neutrophils or neuroendocrine cells, as well as secretion of exosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and clearly written, Exocytosis and Endocytosis: Methods and Protocols is a valuable resource for researchers in the fields of cell biology, neurology, immunology, oncology, and those interested in studying protein trafficking and signal regulation.
Part I. Endocytosis
1. Stoichiometry of Receptors at the Plasma Membrane during Their Endocytosis using Total Internal Reflection Fluorescent (TIRF) Microscopy Live Imaging and Single Molecule Tracking
Laura SalavessaandNathalie Sauvonnet
2. Measuring Endocytosis During Proliferative Cell Quiescence
Claudia Hinze, Kieran McGourty, and Emmanuel Boucrot
3. Measurements of Compensatory Endocytosis by Antibody Internalization and Quantification of Endocytic Vesicle Distribution In Adrenal Chromaffin Cells
Mara Ceridono, Sylvette Chasserot-Golaz, Nicolas Vitale, Stéphane Gasman, and Stéphane Ory
4. Quantitative Methods to Study Endocytosis and Retrograde Transport of Cargo Proteins
Massiullah Shafaq-Zadah, Estelle Dransart, and Ludger Johannes5. High-content Drug Discovery Screening of Endocytosis Pathways
David A. Cardoso, Ngoc Chau, and Phillip J. Robinson
6. Methods for Monitoring Endocytosis in Astrocytes
Maja Potokar, Jernej Jorgačevski, and Robert Zorec
7. Monitoring Activity-Dependent Bulk Endocytosis in Primary Neuronal Culture Using Large Fluorescent Dextrans
Michael A. Cousinand Karen J. Smillie
PART II. Exocytosis
8. Quantitative Flow Cytometry-Based Assays for Measuring Constitutive Secretion
David E. Gordon, Amber S. Shun-Shion, Asral W. Asnawi, and Andrew A. Peden
9. High-throughput Screening for Insulin Secretion Modulators
Michael A. Kalwat
10. Different Approaches to Record Human Sperm Exocytosis
Laila Suhaiman, Karina Noel Altamirano, Alfonsina Morales, and Silvia Alejandra Belmonte
11. Bovine Chromaffin Cells: Culture and Fluorescent Assay for Secretion
Thamou Thahouly, Emeline Tanguy, Juliette Raherindratsara, Marie-France Bader, Sylvette Chasserot-Golaz, Stéphane Gasman, and Nicolas Vitale
12. Measurement of Exocytosis in Genetically Manipulated Mast Cells
Ofir Klein, Nurit P Azouz, and Ronit Sagi-Eisenberg
13. Super-Resolution Microscopy and Particle Tracking Approaches for the Study of Vesicular Trafficking in Primary Neutrophils
Jennifer L. Johnson, Kersi Pestonjamasp, William B. Kiosses, and Sergio D. Catz
14. An Approach to Monitor Exocytosis in White Adipocytes
Ali M. Komai, Man Mohan Shrestha, Saliha Musovic, and Charlotta S. Olofsson
15. Amperometry in Single Cells and Tissue
Damien J. Keating
16. Measurements of Exocytosis by Capacitance Recordings and Calcium Uncaging In Mouse Adrenal Chromaffin Cells
Sébastien Houy, Joana S. Martins, Ralf Mohrmann, and Jakob Balslev Sørensen
17. RUSH-synchronized Secretion to Measure Local Exocytosis
Gaelle Boncompain, Lou Fourriere, Nelly Gareil, and Franck Perez
18. Combining Single Molecule Super-Resolution Imaging Techniques to Unravel the Nanoscale Organization of the Presynapse
Christopher Small, Ramon Martínez-Mármol, Rumelo Amor, Merja Joensuu, and Frederic A. Meunier
19. Induction of Ca2+ Dependent Exocytotic Processes by Laser Ablation of Endothelial Cells
Arsila P. K. Ashraf, Sophia N. Koerdt, Nikita Raj, and Volker Gerke
20. Transmission Electron Microscopy and Tomography on Plasma Membrane Sheets to Study Secretory Docking Franck Delavoie, Cathy Royer, Stéphane Gasman, Nicolas Vitale, and Sylvette Chasserot-Golaz
21. Spatial and Temporal Aspects 0f Exocytosis Studied on the Isolated Plasma Membranes
Ira MilosevicIncludes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
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