Galectins (2^nd Ed., 2nd ed. 2022) Methods and Protocols Methods in Molecular Biology Series, Vol. 2442

This second edition is dedicated to new and updated methodological approaches designed to study galectin function. Chapters examine salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Galectins: Methods and Protocols, Second Eidtion aims to be a useful practical guide to researches to help further their study in this field. 

1.     Galectins: An Ancient Family of Carbohydrate Binding Proteins with Modern Functions

Hans Verkerke, Marcelo Dias-Baruffi, Richard D. Cummings, Connie M. Arthur, and Sean R. Stowell

2.     Cloning, Expression and Purification of Galectins for In Vitro Studies

Paul A Poland, Carol L Kinlough, and Rebecca P Hughey

3.     Purification of Recombinant Galectins Using Affinity Chromatography Methods

Anu Paul, Shang-Chuen Wu, Kashyap R. Patel, Alex D. Ho, Jerry William Lynn Allen, Hans Verkerke, Connie M. Arthur, and Sean R. Stowell

4.     Alkylation of Galectin-1 with Iodoacetamide and Mass Spectrometric Mapping of the Sites of Incorporation

Shang-Chuen Wu, Anu Paul, Richard D. Cummings, Christa L. Feasley, Connie M. Arthur, and Sean R. Stowell

5.     Rapid Detection and Purification of Galectin-3 by the Capture and Release (CaRe) Method

Priyanka D. Kadav, Jared L. Edwards, Jessica Krycia, Purnima Bandyopadhyay, and Tarun K. Dam

Mária Raics, István Timári, László Szilágyi, Hans-Joachim Gabius, and Katalin E. Kövér

7.     Evaluation of Galectin Binding by Surface Plasmon Resonance

Padmaja Mehta-D’souza

8.     Revealing the Identity of Human Galectin-3 as a Glycosaminoglycan-Binding Protein

Jared L. Edwards,  Priyanka D. Kadav, Purnima Bandyopadhyay, and Tarun K. Dam

9.     Examining Galectin Binding Specificity Using Glycan Microarrays

Connie M. Arthur, Lilian C Rodrigues, Marcelo Dias-Baruffi, Harold C. Sullivan, Jamie Heimburg-Molinaro, Dave F. Smith, Richard D. Cummings, and Sean R. Stowell

10.  Mechanism of Mucin Recognition by Lectins - A Thermodynamic Study

Tarun K. Dam, Jared L. Edwards, Priyanka D. Kadav, and C. Fred Brewer

11.  Examination of Whole Cell Galectin Binding by Solid Phase and Flow Cytometric Analysis

Anne Leppänen, Connie M. Arthur, Sean R. Stowell, and Richard D. Cummings

12.  Dissecting Context-Specific Galectin Binding Using Glyco-Engineered Cell Libraries

13.  Method for Identifying Galectin Ligands on Lymphocyte Membrane Glycoproteins

Kashyap R. Patel, Adam W. Barb, and Sean R. Stowell

Dan Hu and Jun Hirabayashi

15.  What Happens if a Human Galectin Enters the Endoplasmic Reticulum?

Tanja J. Kutzner, Alonso M. Higuero, Martina Süßmair, Michael Hingar, Herbert Kaltner, Ingo Lindner, Jürgen Kopitz, José Abad-Rodríguez, Dietmar Reusch, and Hans-Joachim Gabius

16.  Investigation of Galectins in Frozen Tissue and Mammalian Cell Culture Using Confocal Microscopy

17.  Exploring the Galectin Network by Light and Fluorescence Microscopy

Gabriel García Caballero, Joachim C. Manning, Adele Gabba, Donella Beckwith, Forrest G. FitzGerald, Tanja J. Kutzner, Anna-Kristin Ludwig, Herbert Kaltner, Paul V. Murphy, Mare Cudic, and Hans-Joachim Gabius

18.  Molecular Imaging for In Vivo Tracking and Detection of Galectin Binding Partners

Thais Canassa De Leo, Sofia Nascimento dos Santos, Emerson Soares Bernardes, Richard D. Cummings, Sean R. Stowell, and Marcelo Dias-Baruffi

19.  Visualization of Cytosolic Galectin Accumulation Around Damaged Vesicles and Organelles

Ming-Hsiang Hong, I-Chun Weng, Fang-Yen Li, and Fu-Tong Liu

20.  Transcytosis of Galectin-3 in Mouse Intestine

Alena Ivashenka, Christian Wunder, Valerie Chambon, Estelle Dransart, Ludger Johannes, and Massiullah Shafaq-Zadah

21.  Evaluating the Role of Galectins in Clathrin Independent Endocytosis

22.  Examination of Galectin-3-Recruitment into Multivesicular Bodies for Exosomal Secretion

Sebastian Bänfer, Sophie Kutscher, and Ralf Jacob

Chiguang Feng, Mihai Nita-Lazar, Nuria González-Montalbán, Jingyu Wang, Justin Mancini, Sheng Wang, Chinnarajan Ravindran, Hafiz Ahmed, and Gerardo R. Vasta     

Sebastian Schmidt, Herbert Kaltner, and Hans-Joachim Gabius

25.  Examining the Impact of Galectin-9 on Latent HIV Transcription

Opeyemi S. Adeniji, Leila B. Giron, and Mohamed Abdel-Mohsen

26.  Evaluation of the Role of Galectins in Parasite Immunity

Jaclyn Swan, Dhana Sakthivel, Travis Beddoe, Michael Stear, David Piedrafita, and Sarah Preston                                      

27.  Evaluation of the Bactericidal Activity of Galectins

28.  Detection of Phosophatidylserine Exposure on Leukocytes Following Treatment with Human Galectins

Sean R. Stowell, Marcelo Dias-Baruffi, Richard D. Cummings, and Connie M. Arthur

29.  Detection of Reactive Oxygen Species in Human Neutrophils Under Various Conditions of Exposure to Galectin

Lilian Cataldi Rodrigues, Daniel Giuliano Cerri, Cleni M. Marzocchi-Machado, Richard D. Cummings, Sean R. Stowell, and Marcelo Dias-Baruffi

Asmi Chakraborty, Norhan B. B. Mohammed, Angela Bernasconi and Charles J. Dimitroff

31.  Methods for Assessing the Effects of Galectins on Leukocytetrafficking and Clearance

Hannah L Law and Dianne Cooper

32.  Examination of the Contributions of Maternal/Placental-Derived Galectin-1 to Pregnancy Outcome

Sophia Borowski, Nancy Freitag, Iris Urban, Geert Michel, Gabriela Barrientos, and Sandra M. Blois

33.  Method to Study the Role of Galectins in Angiogenesis In Vivo Using the Chick Chorioallantoic Membrane Assay

Kitty CM Castricum and Victor LJL Thijssen

Nadia Bannoud, P. Alfredo García, Julian Gambarte-Tudela, Victoria Sundblad, Alejandro J. Cagnoni, Camila A. Bach, Juan M. Pérez Saez, Ada G. Blidner, Sebastián M. Maller, Karina V. Mariño, Mariana Salatino, Juan P. Cerliani, Gabriel A. Rabinovich, and Diego O. Croci

Kitty CM Castricum and Victor LJL Thijssen

36.  Evaluating Therapeutic Activity of Galectin-1 in Sarcolemma Repair of Skeletal Muscle

Mary L. Vallecillo-Zúniga, Matthew Rathgeber, Daniel Poulson, Braden Kartchner, Jacob Luddington, Hailie Gill, Spencer Hayes, Matthew Teynor, Caleb Stowell, Connie Arthur, Sean Stowell, and Pam M. Van Ry

37.  Exploring the Role of Galectins in Cancer: In Vitro and In Vivo Approaches

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts