Heterologous Expression of Membrane Proteins (2^nd Ed., Softcover reprint of the original 2nd ed. 2016) Methods and Protocols Methods in Molecular Biology Series, Vol. 1432

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer?s disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.

1. Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies

            Ralf-Bernhardt Rues, Erik Henrich, Coilin Boland, Martin Caffrey, and Frank Bernhard

2. Membrane Protein Production in the Yeast, S. cerevisiae

            Stephanie P. Cartwright, Lina Mikaliunaite, and Roslyn M. Bill

3. Membrane Proteins Production in Escherichia coli: Protocols and Rules

            Federica Angius, Oana Ilioaia, Marc Uzan, and Bruno Miroux

4. Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach

            Kiavash Mirzadeh, Stephen Toddo, Morten H.H. Nørholm, and Daniel O. Daley

5. Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins

            Juni Andréll, Patricia C. Edwards, Fan Zhang, Maria Daly, and Christopher G. Tate

6. Membrane Protein Production in Lactococcus lactis for Functional Studies

            Daphne Seigneurin-Berny, Martin S. King, Emiline Sautron, Lucas Moyet, Patrice Catty, François André, Norbert Rolland, Edmund R.S. Kunji, and Annie Frelet-Barrand

7. Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells

            Renato Mancini Astray, Sandra Fernanda Suárez-Patiño, and Soraia Attie Calil Jorge

8. Leishmania tarentolæ as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6

            Lucia Gonzalez-Lobato, Vincent Chaptal, Jennifer Molle, and Pierre Falson

9. Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA

10. Expression of Eukaryotic Membrane Proteins in Pichia pastoris

Includes cutting-edge methods and protocols for the study of recombinant integral membrane

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts