Imaging and Tracking Stem Cells (2^nd Ed., 2nd ed. 2020)
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2150

This fully updated book brings together protocols to arm stem cell biologists with tools and approaches to continue uncovering the intricacies and regulatory mechanisms underlying stem cell biology. Through various models and organ systems, the volume reflects the numerous recent advances in cell lineage and lineage tracking. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Imaging and Tracking Stem Cells: Methods and Protocols, Second Edition is an ideal guide for novices and experts alike who are working to expand our knowledge in the field of stem cells.

1. Molecular Imaging and Tracking Stem Cells in Neurosciences

            Nora Sandu, Thomas Rosemann, and Bernhard Schaller

2. Tracking Germline Stem Cell Dynamics In Vivo in C. elegans Using Photoconversion

3. Long-Term Cell Fate Tracking of Individual Renal Cells Using Serial Intravital Microscopy

            Ina Maria Schiessl, Katharina Fremter, James L. Burford, Hayo Castrop, and Janos Peti-Peterdi

4. Tracking and Imaging of Transplanted Stem Cells in Animals

            Tanmayee Rajeev Chikate and Liping Tang

5. Generation and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes and Endothelial Cells for High Content Screening Purposes

            Tünde Berecz, Mária Husvéth-Tóth, Maxime Mioulane, Béla Merkely, Ágota Apáti, and Gábor Földes

6. Covisualization of Global DNA Methylation/Hydroxymethylation and Protein Biomarkers for Ultra-High Definition Epigenetic Phenotyping of Stem Cells

            Jian Tajbakhsh

7. A Distinctive MRI-Based Absolute Bias Correction Protocol for the Potential Labeling and In Vivo Tracking of Stem Cells in a TBI Mice Model

            Sushanta Kumar Mishra, Subash Khushu, and Gurudutta Gangenahalli

8. Efficient Labeling of Human Mesenchymal Stem Cells Using Iron Oxide Nanoparticles

            Sonali Rawat, Suchi Gupta, Madhusudan Bhat, Amit Kumar Dinda, and Sujata Mohanty

9. Imaging and Tracking Stem Cell Engraftment in Ischemic Hearts by Near-Infrared Fluorescent Protein (iRFP) Labeling

            Xuan Su, Yan Shen, Neal L. Weintraub, and Yaoliang Tang

10. Long-Term Intravital Imaging of Cornea, Skin, and Hair Follicle by Multiphoton Microscope

            Yueh-Feng Wu, Hsin-Yuan Tan, and Sung-Jan Lin

11. Cell Cycle Analysis Using In VivoStaining of DNA Synthesizing Cells

            Petr Páral, Martin Báječný, Filipp Savvulidi, and Emanuel Nečas

12. Metabolic Labeling of Live-Stem Cell for In Vitro Imaging and In Vivo Tracking

            Sang-Soo Han and Sun-Woong Kang

13. Study of Intracellular Cargo Trafficking and Co-Localization in the Phagosome and Autophagy-Lysosomal Pathways of Retinal Pigment Epithelium (RPE) Cells

            J. Arjuna Ratnayaka, Eloise Keeling, and David S. Chatelet

14. Timelapse Video-Microscopy and Single Cell Tracking to Study Neural Cell Behavior In Vitro

            Lucía Paniagua-Herranz, Rosa Gomez-Villafuertes, David de Agustín-Durán, Sergio Gascón, Raquel Pérez-Sen, Esmerilda G. Delicado, María Teresa Miras-Portugal, and Felipe Ortega

15. Multiphoton Microscopy for Non-Invasive and Label-Free Imaging of Human Skin and Oral Mucosa Equivalents

            Gopu Sriram, Sudhaharan Thankiah, and Graham D. Wright

16. Molecular Imaging of Therapeutic Effect of Mesenchymal Stem Cell-Derived Exosomes for Hindlimb Ischemia Treatment

            Kaiyue Zhang and Zongjin Li

17. In Vitro Methods to Simulate Pollution and Photo-Pollution Exposure in Human Skin-Epidermis

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts