Description
Intestinal Stem Cells, 1st ed. 2020
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2171
Coordinator: Ordóñez-Morán Paloma
Language: EnglishSubjects for Intestinal Stem Cells:
Publication date: 07-2021
350 p. · 17.8x25.4 cm · Broché
Publication date: 07-2020
350 p. · 17.8x25.4 cm · Relié
Résumé
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Part I: Characterization, Imaging, and Functional Assays
1. Identification and Isolation of Human LGR5+ Cells Using an Antibody-Based Strategy
Michael K. Dame, Sha Huang, Durga Attili, Jason R Spence, and Justin A. Colacino
2. Immune-Mediated Specific Depletion of Intestinal Stem Cells
Stephen E. Sherman and Judith Agudo
3. Analysis of Aged Dysfunctional Intestinal Stem Cells
Kodandaramireddy Nalapareddy and Hartmut Geiger
4. Strategies for Measuring Induction of Fatty Acid Oxidation in Intestinal Stem and Progenitor Cells
Chia-Wei Cheng, Omer H. Yilmaz, and Maria M. Mihaylova
5. Visualization of Stem Cell Niche by the Fluorescence Lifetime Imaging Microscopy
Irina A. Okkelman, Jens Puschhof, Dmitri B. Papkovsky, and Ruslan I. Dmitriev
6. Generation and Quantitative Imaging of Enteroid Monolayers
Laura E. Sanman, Ina W. Chen, Jake M. Bieber, Curtis A. Thorne, Lani F. Wu, and Steven J. Altschuler
7. Autophagy Detection in Intestinal Stem Cells
Jumpei Asano, Taku Sato, and Toshiaki Ohteki
Part II: Single-Cell Transcriptional Profiling of the Small Intestinal Epithelium
8. Single-Cell Transcriptional Profiling of the Intestinal Epithelium
Claudia Capdevila, Ruben I. Calderon, Erin C. Bush, Kismet Sheldon-Collins, Peter A. Sims, and Kelley S. Yan
9. Single-Cell Studies of Intestinal Stem Cell Heterogeneity during Homeostasis and Regeneration
Maxim Norkin, Claudia Capdevila, Ruben I. Calderon, Tianhong Su, Maria Trifas, Paloma Ordóñez-Morán, and Kelley S. Yan
Part III: Organoids and Applications
10. Large-Scale Production of Recombinant Noggin and R-Spondin1 Proteins Required for the Maintenance of Stem Cells in Intestinal Organoid Cultures
David L. Hacker and Paloma Ordóñez-Morán
11. Primary Intestinal Epithelial Organoid Culture
Tomohiro Mizutani and Hans Clevers
12. In Vivo Human PSC-Derived Intestinal Organoids to Study Stem Cell Maintenance
Simon Vales, Holly M. Poling, Nambirajan Sundaram, Michael A. Helmrath, and Maxime M. Mahe
13. Generation of Knock-Out Gene-Edited Human Intestinal Organoids
Chathruckan Rajendra, Tomas Wald, Kevin Barber, Jason R. Spence, Faranak Fattahi, and Ophir D. Klein
14. Direct Lineage Reprogramming of Mouse Fibroblasts to Acquire the Identity of Fetal Intestine-Derived Progenitor Cells
Shizuka Miura and Atsushi Suzuki
15. Single Molecule RNA FISH in Whole-Mount Organoids
Costanza Borrelli and Andreas E. Moor
16. Specific Gene Expression in Lgr5+ Stem Cells by Using Cre-Lox Recombination
Pierre Dessen, Joerg Huelsken, and Paloma Ordóñez-Morán
17. Generating and Utilizing Murine Cas9-Expressing Intestinal Organoids for Large Scale Knockout Genetic Screening
Hossein Kashfi, Nicholas Jinks, and Abdolrahman Shams Nateri
Part IV: In Vivo Models
18. Mouse Model for Sporadic Mutation of Target Alleles to Understand Tumor Initiation and Progression and Stem Cell Dynamics
Theresa Nguyen, Elise Manalo, Taryn Kawashima, and Jared M. Fischer
19. Hemagglutinating Virus of Japan Envelop (HVJ-E)-Guided Gene Transfer to the Intestinal Epithelium
Masamichi Imajo
20. An Intrasplenic Injection Model for the Study of Cancer Stem Cells Seeding Capacity
Caroline Dafflon, Albert Santamaría-Martínez, and Paloma Ordóñez-Morán
21. Organoid Derivation and Orthotopic Xenotransplantation for Studying Human Intestinal Stem Cell Dynamics
Shinya Sugimoto, Masayuki Fujii, and Toshiro Sato
22. Advanced Colorectal Cancer Orthotopic Patient-Derived Xenograft Models for Cancer and Stem Cell Research
Irene Chicote, Juan Antonio Cámara, and Héctor G. Palmer
23. Modeling Colorectal Cancer Progression through Orthotopic Implantation of Organoids
Felipe de Sousa e Melo, Jonathan M. Harnoss, Noelyn Kljavin, Ryan Scott, Catherine Sohn, Kevin G. Leong, and Frederic J. de Sauvage
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
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