Isotope Labeling of Biomolecules – Labeling Methods
Methods in Enzymology Series

Labeling in Prokarya1. Robust High Yield Methodologies for 2H and 2H/15N/13C Labelling of Proteins for Structural Investigations using Neutron Scattering and NMR Anthony P. Duff, Karyn L. Wilde, Agata Rekas, Vanessa Lake and Peter J. Holden2. Protein labeling in Escherichia coli with 2H, 13C and 15NJ. Todd Hoopes, Margaret A. Elberson, Renae J. Preston, Prasad T. Reddy and Zvi Kelman3. Escherichia Coli Auxotroph Host Strains for Amino Acid-Selective Isotope Labeling of Recombinant Proteins Myat T. Lin, Risako Fukazawa, Yoshiharu Miyajima-Nakano, Shinichi Matsushita, Sylvia K. Choi, Toshio Iwasaki and Robert B. Gennis4. 19F-Modified Proteins and 19F-Containing Ligands as Tools in Solution NMR Studies of Protein InteractionsNaima G. Sharaf and Angela M. Gronenborn5. Biopolymer Deuteration For Neutron Scattering and Other Isotope-Sensitive TechniquesRobert A. Russell, Christopher J. Garvey, Tamim A. Darwish, L. John R. Foster and Peter J. Holden6. Production of Bacterial Cellulose with Controlled Deuterium-Hydrogen Substitution for Neutron Scattering StudiesHugh O’Neill, Riddhi Shah, Barbara Evans, Junhong He, Sai Venkatesh Pingali, Shishir P.S. Chundawat, A. Daniel Jones, Paul Langan, Brian H. Davison and Volker Urban7. Isotopic Labeling of Proteins in Halobacterium SalinarumThomas E. Cleveland and Zvi Kelman8. Amino Acid Selective Unlabeling in Protein NMR SpectroscopyChinmayi Prasanna, Abhinav Dubey and Hanudatta S. Atreya

Labeling in Eukarya9. Isotope Labeling of Eukaryotic Membrane Proteins in Yeast for Solid-State NMR Ying Fan, Sanaz Emami, Rachel Munro, Vladimir Ladizhansky and Leonid S. Brown10. Development of Approaches for Deuterium Incorporation in PlantsBarbara R. Evans and Riddhi Shah11. Isotope labeling of proteins in insect cellsLukasz Skora, Binesh Shrestha and Alvar D. Gossert12. Effective Isotope Labeling of Proteins in a Mammalian Expression SystemMallika Sastry, Carole A. Bewley and Peter D. Kwong

In Vitro Labeling13. Escherichia Coli Cell-Free Protein Synthesis and Isotope Labeling of Mammalian ProteinsTakaho Terada and Shigeyuki Yokoyama14. Rapid Biosynthesis of Stable Isotope Labeled Peptides from a Reconstituted in Vitro Translation System for Targeted Proteomics Feng Xian, Shuwei Li and Siqi Liu15. Labeling of Membrane Proteins by Cell-Free ExpressionAisha Laguerre, Frank Löhr, Frank Bernhard and Volker Dötsch16. Selective Amino Acid Segmental Labeling of Multi-Domain ProteinsErich Michel and Frédéric H.-T. Allain17. Labeling Monosaccharides with Stable IsotopesWenhui Zhang, Shikai Zhao and Anthony S. Serianni

• Contains contributions from leading authorities in the field of isotope labeling of biomolecules
• Informs and updates on the latest developments in the field
• Provides comprehensive information on stable isotope labeling methods and applications for biomolecules