Lectin Purification and Analysis, 1st ed. 2020
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2132

This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectins?these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.

Preface…
Table of Contents…
Contributing Authors…

1. Structural database for Lectins and the UniLectin Web Platform
François Bonnardel, Serge Perez, Frédérique Lisacek, and Anne Imberty 

2. Purification of Sugar-Binding Peptides from L-Type Lectins
Kazuo Yamamoto

3. Recombinant Expression and Purification of Animal Intracellular L-Type Lectins
Tadashi Satoh and Koichi Kato

4. Frontal Affinity Chromatography: A Highly Suitable Retardation Phenomenon-Based research Tool for Analyzing Weak Interactions between Biomolecules
Kenichi Kasai

5. Metazoan Soluble b-Galactoside-Binding Lectins, Galectins: Methods for Purification, Characterization of their Carbohydrate Binding Specificity, and Probing their Ligands
Guillaume St-Pierre, Ann Rancourt, and Sachiko Sato

6. Expression, S-Nitrosylation, and Measurement of S-Nitrosylation Ratio of Recombinant Galectin-2
Mayami Tamura and Yoichiro Arata

7. Expression and Purification of Full-Length and Domain-Fragment Recombinant Pentraxin 3 (PTX3) Proteins from Mammalian and Bacterial Cells
Kenji Daigo and Takao Hamakubo

8. Identification of Siglec Cis-Ligands by Proximity Labeling
Amin Alborzian Deh Sheikh, Chizuru Akatsu, and Takeshi Tsubata

9. Preparation of Recombinant Siglecs and Identification of their Ligands
Lan-Yi Chang, Penk Yeir Low, Deepa Sridharan, Kaia Gerlovin, and Takashi Angata

10. Purification, Quantification, and Functional Analysis of Collectins
Katsuki Ohtani and Nobutaka Wakamiya

11. Selectin-Binding Assay by Flow-Cytometry
Keiichiro Sakuma and Reiji Kannagi

12. Direct Binding Analysis between C-Type Lectins and Glycans Using Immunoglobulin-Receptor Fusion Proteins
Miyuki Watanabe, Zakaria Omahdi, and Sho Yamasaki

13. Expression and Characterization of Hyaluronan Binding Protein Involved in Hyaluronan Depolymerization: HYBID, alias KIAA1199 and CEMIP
Susana de Vega, Hiroyuki Yoshida, and Yasunori Okada

14. Paracoccin: Purification and validation of its Lectin and Enzymatic Properties
Nayla de Souza Pitangui, Fabrício Freitas Fernandes, Relber Aguiar Gonçales, and Maria Cristina Roque-Barreira

15. In Vitro Mannosidase Assay of EDEMs: ER Degradation-Enhancing b-Mannosidase-Like Proteins
Nobuko Hosokawa

16. Galactose-Specific, Hemolytic Lectin CEL-III from Cucumaria echinata
Tomomitsu Hatakeyama

17. Simple and Rapid Detection of Glycoforms by “Lectin Inhibition” Assay
Kyoka Hoshi, Yasuhiro Hasimoto, and Hiromi Ito

18. Glycoform-Specific Visualization in Immunohistochemistry by “Lectin Inhibition”
Hiromi Ito, Kyoka Hoshi, Yasuhiro Hashimoto, and Takashi Honda

19. Botulinum Hemagglutinin: Critical protein for Adhesion and Absorption of Neurotoxin Complex in Host Intestine
Sho Amatsu and Yukako Fujinaga

20. Functional Analysis of Botulinum Hemagglutinin (HA)
Takuhiro Matsumura and Yukako Fujinaga

21. Purification and Functional Characterization of the Effects on Cell Signaling of Mytilectin: A Novel β-Trefoil Lectin from Marine Mussels
Yuki Fujii, S.M. Abe Kawsar, Imtiaj Hasan, Hideaki Fujita, Marco Gerdol, and Yasuhiro Ozeki

22. Lectin-Type Ubiquitin Ligase Subunits: Fbs Proteins and their Applications for Use
Yukiko Yoshida

23. F-Type Lectins: Structure, Function, and Evolution
Gerardo R. Vasta and Chiguang Feng

24. Purification and Biochemical Characterization of Selected F-Type Lectins
Chiguang Feng and Gerardo R. Vasta

25. LecA (PA-IL): A Galactose-Binding Lectin from Pseudomonas aeruginosa
Sakonwan Kuhaudomlarp, Emilie Gillon, Annabelle Varrot, and Anne Imberty

26. Glycan Recognition and Application of P-Type Lectins
Kei Kiriyama and Kohji Itoh

27. Purification and Assays of Tachylectin-5
Shun-ichiro Kawabata and Toshio Shibata

28. Preparation of Soluble Malectin and its Tetramer
Sheng-Ying Qin, Dan Hu, and Kazuo Yamamoto

29. Calnexin/Calreticulin and Assays Related to N-Glycoprotein Folding In Vitro
Yoshito Ihara, Midori Ikezaki, Maki Takatani, and Yukishige Ito

30. Purification and Assays of Tachylectin-2
Shun-ichiro Kawabata and Toshio Shibata

31. Purification and Assays of Tachycitin
Shun-ichiro Kawabata and Toshio Shibata

32. Methods for Purifying Datura stramonium Agglutinin and Producing Recombinant Agglutinin Protein in a Heterologous Plant Host
Suguru Oguri

33. ZG16p, An Animal Homologue of Plant β-Prism Fold Lectins: Purification Methods of Natural and Recombinant ZG16p and Inhibition Assay of Cancer Cell Growth using ZG16p
Akiko Mito, Kaori Kumazawa-Inoue, and Kyoko Kojima-Aikawa

34. ArtinM: Purification and Evaluation of Biological Activities
Thiago Aparecido da Silva, Patrícia Kellen Martins Oliveira-Brito, Sandra Maria de Oliveira Thomaz, and Maria Cristina Roque-Barreira

35. Bacterial Expression of Rhamnose-Binding Lectin from Catfish Eggs
Shigeki Sugawara, Takeo Tatsuta, and Masahiro Hosono

36. A Bioassay for Determining Symbiotic Zooxanthellae Shape Control Using Lectin SLL-2 from the Octocoral Sinularia lochmodes
Mitsuru Jimbo, Ryota Takeuchi, and Mayu Yoshino

37. MIC4 from Toxoplasma gondii: A Lectin Acting as a Toll-Like Receptor Agonist
Flávia Costa Mendonça-Natividade, Rafael Ricci-Azevedo, and Maria Cristina Roque-Barreira

38. Production and Characterization of MIC1: A Lectin from Toxoplasma gondii
Flávia Costa Mendonça-Natividade, Rafael Ricci-Azevedo, Sandra Maria de Oliveira Thomaz, and Maria Cristina Roque-Barreira

39. Affinity Labeling and Purification of Plant Chitin-Binding LysM-Receptor with Chitin Octasaccharide Derivatives
Tomonori Shinya, Naoto Shibuya, and Hanae Kaku

40. Purification of GNA-Related Lectins from Natural Sources
Els J.M. Van Damme

41. Expression, Purification, and Applications of the Recombinant Lectin PVL from Psathyrella velutina  Specific for Terminal N-Acetyl-Glucosamine
Oriane Machon and Annabelle Varrot

42. Yeast Flocculin: Methods for Quantitative Analysis of Flocculation in Yeast Cells
Hiromi Maekawa and Kaoru Takegawa

43. Pleurotus cornucoiae Mycelial Lectin (PCL-M): Purification and Detection of the Acitivty on Mycelial Surface
Suguru Oguri

44. Expression and Purification of a Human Pluripotent Stem Cell-Specific Lectin Probe, rBC2LCN
Hiroaki Tateno

45. Preparation of Fluorescent Recombinant Shiga Toxin-B-Subunit and its Application to Flow Cytometry
Toshiyuku Yamaji

46. LecB, A High Affinity Soluble Fucose-Binding Lectin from Pseudomonas aeruginosa
Emilie Gillon, Annabele Varrot, and Anne Imberty

47. Sialoglycovirology of Lectins: Sialyl Glycan Binding of Enveloped and Non-Enveloped Viruses
Nongluk Sriwilaijaroen and Yasuo Suzuki

48. Hemagglutinin Inhibitors are Potential Future Anti-Influenza Drugs for Mono- and Combination-Therapies
Nongluk Sriwilaijaroen and Yasuo Suzuki

49. Preparation and Detection of Glycan Binding Activity of Influenza Virus
Shin-ichi Nakakita, Nongluk Sriwilaijaroen, Yasuo Suzuki, and Jun Hirabayashi

50. Screening for Components/Compounds with Anti-Rotavirus Activity: Detection of Interaction between Viral Spike Proteins and Glycans
Keita Yamada, Junko Nio-Kobayashim and Mizuho Inagaki

51. ELISA-Based Methods to Detect and Quantify Norovirus Virus-Like Particle Attachment to Histo-Blood Group Antigens
Haruko Shirato

52. FAM3B/PANDER-Like Carbohydrate-Binding Domain in a Glycosyltransferase, POMGNT1
Hiroshi Manya, Maoyuki Kuwabaram Ryuichi Kato, and Tamao Endo

53. Mannose-Specific Oyster Lectin CGL1
Hideaki Unno and Tomomitsu Hatakeyama

54. Receptor Binding Assays of Enterovirus D68
Tadatsugu Imamura, Michiko Okamoto, and Hitoshi Oshitani

55. Large-Scale Expression and Purification of Mumps Virus Hemagglutinin-Neuraminidase for Structural Analyses and Glycan-Binding Assays
Marie Kubota and Takao Hashiguchi

56. Purification of AJLec: A Novel Galactose-Specific Lectin, from the Sea Anemone Anthopleura japonica
Hideaki Unno

57. Annexin Lectins: Ca2+-Dependent Heparin-Binding Activity, Phosphatidylserine-Binding Activity, and Anticoagulant Activity
Moeka Nakayama, Miyuki Tsunooka-Ohta, and Kyoko Kojima-Aikawa

58. Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans
Therese Wohlschlager, Alexander Titz, Markus Künzler, and Annabelle Varrot

59. The OAAH Family: Anti-Influenza Virus Lectins
Yuichiro Sato, Makoto Hirayama, Kinjiro Morimoto, and Kanji Hori

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts