Lysosomes, 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1594

This volume provides laboratory protocols essential for studies on lysosomal biology. Chapters aim to guide researchers in their exploration of lysosomes, both under normal conditions and in pathological processes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lysosome: Methods and Protocols aims to provided protocols that will guide and inspire further research and generate new insights into this fascinating organelle.

1.      SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS
Melanie Thelen, Dominic Winter, Thomas Braulke, and Volkmar Gieselmann

2.      Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics
 Mesut Bilgin, Jesper Nylandsted, Marja Jäättelä, and Kenji Maeda

3.      Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins
Elmira Tokhtaeva, Olga A. Mareninova, Anna S. Gukovskaya, and Olga Vagin

5.      Microscopic Analysis of Lysosomal Membrane Permeabilization
Ana Maria Vilamill Giraldo, Karin Öllinger, and Vesa Loitto

  6.      Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets
Frederik W. Lund and Daniel Wüstner

7.      Preparation of a Two-Photon Fluorescent Probe for Imaging H₂O₂ in Lysosomes in Living Cells and Tissues
Mingguang Ren, Beibei Deng, Xiuqi Kong, Yonghe Tang, and Weiying Lin

8.      Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery
Takanobu Otomo and Tamotsu Yoshimori

9.      Delivery of Cargo to Lysosomes using Gneosomes
Kristina M. Hamill, Ezequiel Wexselblatt, Wenyong Tong, Jeffrey D. Esko, and Yitzhak Tor

10.  Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles
Camilla Lööv and Anna Erlandsson  

11.  Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells
Ida Eriksson, Karin Öllinger, and Hanna Appelqvist

12.  Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
Anna L. Södergren and Sofia Ramström

14.  Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates
Neil D. Rawlings

15.  Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex
Giuseppina Di Fruscio, Sandro Banfi, Vincenzo Nigro, and Andrea Ballabio

16.  Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms
Yoko Hashimoto

18.  Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi
Jyotsna Vermaa, Sunita Bijarnia-Mahaya, and Ishwar C. Vermaa

19.  Lysosomal Biology in Cancer
Colin Fennelly and Ravi Amaravadi

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts