Macro-Glycoligands, Softcover reprint of the original 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1367

This volume details methods and protocols for the synthesis and characterization of glycopolymers and their biomedical applications. Chapters are divided into three parts covering synthesis and characterization of glycopolymers; glycopolymer-nanoparticle conjugates; and surface immobilized glycopolymers.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Macro-Glycoligands: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Part I: Synthesis and Characterization of Glycopolymers

1. Synthesis of Chain-end Functionalized Glycopolymers via Cyanoxyl Mediated Free Radical Polymerization (CMFRP)

Valentinas Gruzdys, Jinshan Tang, Elliot Chaikof, Xue-Long Sun

2. Protecting-group-free Synthesis of Well-defined Glycopolymers Featuring Negatively Charged Oligosaccharides

Luca Albertin

3. Glycopolymers Prepared by Ring-opening Metathesis Polymerization Followed by Glycoconjugation Using a Triazole Forming ‘Click’ Reaction

Ronald Okoth and Amit Basu

4. Protecting-group-free Synthesis of Glycopolymers and Their Binding Assay with Lectin and Influenza Virus

Tomonari Tanaka, Tadanobu Takahashi, and Takashi Suzuki

5. Carbohydrate-based Initiators for the Cationic Ring-opening Polymerization of 2-Ethyl-2-oxazoline

Christine Weber,Michael Gottschaldt, Richard Hoogenboom, Ulrich S. Schubert

6. Heterofunctional Glycopolypeptides by Combination of Thiol-ene Chemistry and NCA Polymerization

Kai-Steffen Krannig and Helmut Schlaad

7. Preparation of Proteoglycan Mimetic Graft Copolymers

Matt J. Kipper and Laura W. Place

Part II: Glycopolymer Nanoparticle Conjugates

8. Galactosylated Polymer Nano-objects by Polymerization-Induced Self-Assembly, Potential Drug Nanocarriers

Mona Semsarilar, Irene Canton, and Vincent Ladmiral

9. Synthetic Approach to Biotinylated Glyco-Functionalized Quantum Dots: A New Fluorescent Probes for Biomedical Applications

Christian K. Adokoh, James Darkwa, and Ravin Narain

10. Surface Modification of Polydivinylbenzene Microspheres with a Fluorinated Glycopolymer using Thiol-Halogen Click Chemistry

Wentao Song and Anthony M. Granville

11. Glycopolymer-grafted Polymer Particles for Lectin Recognition

Michinari Kohri, Tatsuo Taniguchi, and Keiki Kishikawa

12. Synthesis of Non-spherical Glycopolymer-decorated Nanoparticles: Combing Thiol-ene with Catecholic Chemistry

Xiao Li, Weidong Zhang,and  Gaojian Chen

13. Synthetic Approach To Glycopolymer Base Nanoparticle Gold(I) Conjugate: A New Generation Of Therapeutic Agents

Christian K. Adokoh, James Darkwa, and Ravin Narain

14. Multivalent Glycopolymer-coated Gold Nanoparticles

Sarah-Jane Richards, Caroline I. Biggs, and Matthew I. Gibson

Part III: Surface Immobilized Glycopolymers

15. Modulation of Multivalent Protein Binding on Surfaces by Glycopolymer Brush Chemistry

Kai Yu, A. Louise Creagh,  Charles A. Haynes, Jayachandran N. Kizhakkedathu

16. Oriented Immobilized Sialyloligo-Macroligand Microarray

Satya Nandana Narla and Xue-Long Sun

17. Glycocalyx Remodeling with Glycopolymer-based Proteoglycan Mimetics

Mia L. Huang, Raymond A. A. Smith, Greg W. Trieger, and Kamil Godula

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts