Description
Measuring Gene Expression
THE BASICS (Garland Science) Series
Author: Avison Matthew
Language: English
Subjects for Measuring Gene Expression:
Keywords
DNA Molecule; cell; DNA Polymerase; extract; AMV Reverse Transcriptase; transcriptional; Primary IgG; initiation; DNA Polymerase Enzyme; transfer; DNA Polymerase Activity; buffer; DNA Bind Protein; rna; Thermostable DNA Polymerase; molecule; Transcriptional Initiation; cdna; Tester cDNA; production; cDNA Pool; RNA Polymerase; Driver cDNA; SDS Page Dimension; Data Sets; SDS Page Gel; PCR Product; DNA Probe; DNA Contamination; RNase Free Water; Test Protein; PCR Reaction; DNA Ligase; LacZ Activity; Alternative Sigma Factor
328 p. · 17.4x24.6 cm · Paperback
Description
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In the post-genomic age, much biomedical research looks at when, where, and at what level genes are expressed. Measuring Gene Expression is an all-in-one introduction to the main methods of measuring gene expression, including RT-PCR, differential display, RNA interference, reporter genes, microarrays, and proteomics, as well as a section on RNA isolation and analysis. There is an overview of each method: its pros and cons, sample preparation, sources of error, and data interpretation.
1. Gene Expression and its Control 2. Isolation and Analysis of RNA 3. Hybridization-based Methods for Measuring Transcript Levels 4. PCR-based Methods for Measuring Transcript Levels 5. Differential Display, Subtractive Hybridization, Amplification Suppression and SAGE Techniques for Measuring Gene Expression 6. Measuring Gene Expression Using Reporter Gene Assays 7. Analysis of the Proteome 8. Statistical Analysis of Gene Expression Data
