Metabolic Flux Analysis in Eukaryotic Cells, 1st ed. 2020
Methods and Protocols
Methods in Molecular Biology Series, Vol. 2088

Language: English

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Metabolic Flux Analysis in Eukaryotic Cells
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370 p. · 17.8x25.4 cm · Paperback

158.24 €

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Metabolic Flux Analysis in Eukaryotic Cells
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Support: Print on demand
This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.

Preface…
Table of Contents…
Contributing Authors…

1. Determination of Isotopologue and Tandem Mass Isotopologue Fractions using Gas Chromatography Chemical Ionization Time of Flight Mass Spectrometry: Methodology and Uncertainty of Measurement
Teresa Mairinger and Stephan Hann

2. Non-Targeted Mass Isotopolome Analysis using Stable Isotope Patterns to Identify Metabolic Changes
Christian-Alexander Dudek, Lisa Schlicker, and Karsten Hiller

3. Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13C Meabolic Flux Analysis
Damini Jaiswal, Anjali Mittal, Deepak Nagrath, and Pramod P. Wangikar

4. Deuterium Tracing to Interrogate Compartment-Specific NAD(p)H Metabolism in Cultured Mammalian Cells
Esther W. Lim, Seth J. Parker, and Christian M. Metallo

5. Large-Scale Profiling of Cellular Metabolic Activities using Deep 13C Labeling Medium
Nina Grankvist, Jeramie D. Watrous, Mohit Jain, and Roland Nilsson

6. Analyzing the Metabolism of Metastases in Mice
Patricia Altea-Manzano, Dorien Broekaert, João A.G. Duarte, Juan Fernández-García, Mélanie Planque, and Sarah-Maria Fendt

7. Robusy Analytical methods for the Accurate Quantification of the Total Biomass Composition of Mammalian Cells
Diana Széliová, Harald Schoeny, Špela Knez, Christina Troyer, Cristina Coman, Evelyn Rampler, Gunda Koellensperger, Robert Ahrends, Stephen Hann, Nicole Borth, Jürgen Zanghellini, and David E. Ruckerbauer

8. Quantifying Nitric Oxide Flux Distributions
Darshan M. Sivaloganathan, Xuanqing Wan, and Mark P. Brynildsen

9. SWATH: A Data-Independent Tandem Mass Spectrometry Method to Quantify 13C Enrichment in Cellular Metabolites and Fragments
Damini Jaiswal and pramod P. Wangikar

10. Quantifying Metabolic Transfer Mediated by Extracellular Vesicles using Exo-MFA: An Integrated Empirical and COmputational Platform
Abhinav Achrejal, Noah Meurs, and Deepak Nagrath

11. Comparative Metabolic Network Flux Analysis to Identify Differences in Cellular Metabolism
Sarah McGarrity, Sigurður T. Karvelsson, Ólafur E. Sigurjónsson, and Óttar Rolfsson

12. Software Supporting a Workflow of Quantitative Dynamic Flux Maps Estimation in Central Metabolism from SIRM Experimental Data
Vitaly A. Selivanov, Silvia Marin, Josep Tarragó-Celada, Andrew N. Lane, Richard M. Higashi, Teresa W-M Fan, Pedro de Atauri, and Marta Cascante

13. Inferring Metabolic Flux from Time-Course Metabolomics
Scott Campit and Sriram Chandrasekaran

14. Using Metabolic Network Reconstructions to Predict Drug Targets and Off-Target Effects
Kristopher Rawls, Bonnie V. Dougherty, and Jason Papin

15. Single-Cell Digital Twins for Cancer Preclinical Investigation
Marzia Di Filippo, Chiara Damiani, Marco Vanoni, Davide Maspero, Giancarlo Mauri, Lilia Alberghina, and Dario Pescini

16. Supply and Demand Analysis of Autophagy
André du Toit, Ben Loos, and Jan Hendrik S. Hofmeyr

17. Thermodynamic Approaches in Flux Analysis
Sabine Peres and Vincent Fromion


Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts