Microbial Carotenoids, 1st ed. 2018
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1852

In this volume, expert researchers in the field detail the most up-to-date methods commonly used to study and produce carotenoids. These include methodson the manipulation and metabolic engineering of carotenoid producing microalgae and bacteria, including Corynebacterium glutamicum, Rhodopseudomonas palustris and radio-tolerant bacteria; in addition to fungi, as the beta-carotene producing Blakeslea trispora and Mucor circinelloides or the lycopene producing Blakeslea trispora; and the heterobasidiomycetous yeast producing xanthophylls Xanthophyllomyces dendrorhous (Phaffia rhodozyma) and the engineered yeast Pichia pastoris. Additionally, three overview chapters on the advancement of Biotechnology and carotenoid production are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Microbial Carotenoids: Methods and Protocols provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms, aiming to ensure successful results in the further study of this vital field.

1. Advancement of Biotechnology by Genetic Modifications

Arnold L. Demain and Sergio Sanchez

2. Carotenoids Production: A Healthy and Profitable Industry

Carlos Barreiro and José-Luis Barredo

3. Carotenoids: From Plants to Food and Feed Industries

Panagiota Langi, Sotirios Kiokias, Theodoros Varzakas, and Charalampos Proestos

4. Express Analysis of Microalgal Secondary Carotenoids by TLC and UV-Vis Spectroscopy

Galina S. Minyuk and Alexei E. Solovchenko

5. Batch Cultivation for Astaxanthin Analysis Using the Green Microalga Chlorella zofingiensis under Multitrophic Growth Conditions

Jin Liu

6. Preparative Recovery of Carotenoids from Microalgal Biomass

María del Carmen Cerón García, Cynthia Victoria González López, José María Fernández Sevilla, and Emilio Molina Grima

7. Adaptive Laboratory Evolution for Enhanced Carotenoid Production in Microalgae

8. Carotenoid Production by Recombinant Corynebacterium glutamicum: Strain Construction, Cultivation, Extraction and Quantification of Carotenoids and Terpenes

Nadja A. Henke, Jonas Frohwitter, Petra Peters-Wendisch, and Volker F. Wendisch

9. Rapid and Selective Screening Method for Isolation and Identification of Carotenoid-producing Bacteria

Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda

10. Purification and Identification of Astaxanthin and its Novel Derivative Produced by Radio-tolerant Sphingomonas astaxanthinifaciens

Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda

11. Screening, Isolation, and Identification of Zeaxanthin-producing Bacteria

Dalal Asker, Tarek S Awad, Teruhiko Beppu, and Kenji Ueda

12. Synthesis of Carotenoids of Industrial Interest in the Photosynthetic Bacterium Rhodopseudomonas palustris:Bioengineering and Growth Conditions

Eric Giraud, Laure Hannibal, Clémence Chaintreuil, Joël Fardoux, and André Verméglio

Francisco E. Nicolás, María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Sergio López-García, Eusebio Navarro, Santiago Torres-Martínez, and Victoriano Garre

14. Expression Vectors and Gene Fusions for the Directed Modification of the Carotenoid Biosynthesis Pathway in Mucor circinelloides

Enrique A. Iturriaga, María Isabel Alvarez, Arturo P. Eslava, and Tamás Papp

Sonia Martínez-Cámara, Sara Rubio, Hannah del Río, Marta Rodríguez-Sáiz, and José Luis Barredo

16. HPLC Analysis of Carotenoids in Neurosporaxanthin-producing Fungi

Dámaso Hornero-Méndez, M. Carmen Limón, and Javier Avalos

17. Extraction and Analysis of Carotenes and Xanthophylls Produced by Xanthophyllomyces dendrorhous

Carlos García-Estrada, Katarina Kosalková, and Isabel-Clara Sánchez-Orejas

18. Isolation and Selection of New Astaxanthin Producing Strains of Phaffia rhodozyma

Diego Libkind, Martín Moliné, and Fernando Colabella

Patricia Veiga-Crespo, José Miguel Araya-Garay, and Tomás G. Villa

20. Isolation and Characterization of Extrachromosomal Double-Stranded RNA Elements from Carotenogenic Yeasts

Marcelo Baeza, María Fernández-Lobato, Jennifer Alcaíno, and Víctor Cifuentes

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts