Molecular Motors (2^nd Ed., Softcover reprint of the original 2nd ed. 2018) Methods and Protocols Methods in Molecular Biology Series, Vol. 1805

This volume expands on the previous edition with a more extensive look at molecular motors and their roles in muscle contractions, vesicle transport, flagellar beating, chromosome segregation, and DNA replication and repair. The chapters in this book are divided into three parts: Part One looks at membrane motors, such as the bacterial flagellar rotary motor; Part Two discusses cytoskeletal motors, such as kinesin and myosin; and Part Three talks about nucleic acid motors, such as DNA polymerases, helicases, and nucleosome remodelers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Preface…

Table of Contents…

Contributing Authors…

1. Cellular and Nuclear Forces: An Overview

Bidisha Sinha, Arikta Biswas, and Gautam V. Soni

Part I Membrane Motors

2. The Bacterial Flagellar Rotary Motor in Action

Nicholas J.S. Delalez and Judith P. Armitage

3. Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase

Ganna O. Krasnoselska and Thomas Meier

Part II Cytoskeletal Motors

4. Using Microfluidics Single Filament Assay to Study Formin Control of Actin Assembly

Guillaume Romet-Lemonne, Bérengère Guichard, and Antoine Jégou

5. Engineering Synthetic Myosin Filaments using DNA Nanotubes

Ruth F. Sommese and Sivaraj Sivaramakrishnan

6. Direct Imaging of Walking Myosin V by High-Speed Atomic Force Microscopy

Noriyuki Kodera and Toshiro Ando

7. High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates

Keith J. Mickolajczyk and William O. Hancock

8. Multicolor Tracking of Molecular Motors at Nanometer Resolution

Sara Wichner and Ahmet Yildiz

9. High-Speed Optical Tweezers for the Study of Single Molecular Motors

L. Gardini, A. Tempestini, F.S. Pavone, and M. Capitanio

10. Determining Stable Single Alpha Helical (SAH) Domain Properties by Circular Dichroism and Atomic Force Microscopy

Matthew Batchelor, Marcin Wolny, Marta Kurzawa, Lorna Dougan, Peter J. Knight, and Michelle Peckham

Part III Nucleic Acids Motors

11. The Role of Supercoiling in the Motor Activity of RNA Polymerases

Annick Lesne, Jean-Marc Victor, Edouard Bertrand, Eugenia Basyuk and Maria Barbi

12. Single-Molecule FRET Analysis of Replicative Helicases

Seung-Jae Lee, Salman Syed, and Taekjip Ha

13. Recombinases and Related Proteins in the Context of Homologous Recombination Analyzed by Molecular Microscopy

Pauline Dupaigne, Eliana Moreira Tavares, Olivier Piétrement, and Eric Le Cam

14. DNA Organization and Segregation Super-Resolved

Diego I. Cattoni, Jean-Bernard Fiche, Antoine Le Gall, and Marcelo Nollmann

15. Electrophoretic Analysis of the DNA Supercoiling Activity of DNA Gyrase

Belén Martínez-García, Antonio Valdés, Joana Segura, Ofelia Díaz-Ingelmo, and Joaquim Roca

16. Single Molecule Angular Optical Trapping for Studying Transcription under Torsion

Jie Ma, Chuang Tan, and Michelle D. Wang

17. Anisotropy-Based Nucleosome Repositioning Assay

Koan Briggs, Gada Al-Ani, Allen Eastlund, and Christopher J. Fischer

Johanna Ludwigsen, Nicola Hepp, Henrike Klinker, Sabrina Pfennig, and Felix Mueller-Planitz

19. Measuring Unzipping and Rezipping of Single Long DNA Molecules with Optical Tweezers 

Damian J. delToro and Douglas E. Smith

20. Single-Molecule Measurements of Motor-Driven Viral DNA Packaging in Bacteriophages Phi29, lambda, and T4 with Optical Tweezers

Nicholas Keller, Damian J. delToro, and Douglas E. Smith

21. Methods for Single Molecule Sensing and Detection Using Bacteriophage Phi29 DNA Packaging Motor

Farzin Haque, Hui Zhang, Shaoying Wang, Chun-Li Chang, Cagri Savran, and Peixuan Guo