Mouse Embryogenesis, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1752

This volume explores protocols for identifying mutant mice and characterizing parts of their anatomical, functional, cellular, and molecular phenotypes. The chapters in this book look at anatomical and functional phenotyping using quantitative imaging, isolation of specific embryonic cell types for cell culture, analysis of gene expression, and how to define chromatin structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Mouse Embryogenesis: Methods and Protocols is a valuable resource for experimentalists interested in discovering new aspects of embryogenesis control, organ function, and the origin of disease.

Preface…

Table of Contents…

Contributing Authors…

1. Mouse Genotyping

Sandra Vuong and Paul Delgado-Olguin

2. Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac

Anna R. Roy and Paul Delgado-Olguin

3. In Vivo Evaluation of the Cardiovascular System of Mouse Embryo and Fetus Using High Frequency Ultrasound

Yu-Qing Zhou, Lindsay S. Cahill, and John G. Sled

4. Dynamic Imaging of Mouse Embryos and Cardiodynamics in Static Culture

Andrew L. Lopez, III and Irina V. Larina

5. In Vivo Imaging of the Mouse Reproductive Organs, Embryo Transfer, and Oviduct Cilia Dynamics Using Optical Coherence Tomography

Shang Wang and Irina V. Larina

6. Live Imaging of Fetal Intra-Abdominal Organs Using Two-Photon Laser-Scanning Microscopy

Yuhki Koike, Bo Li, Yong Chen, Hiromu Miyake, Carol Lee, Lijun Chi, Richard Wu, Mikihiro Inoue, Keiichi Uchida, Masato Kusunoki, Paul Delgado Olguin, and Agostino Pierro

7. Embrionary Mouse Cardiac Fibroblast Isolation

Alejandra Garate-Carrillo and Israel Ramirez-Sanchez

8. Explant Culture for Studying Lung Development

Behzad Yeganeh, Claudia Bilodeau, and Martin Post

9. Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting

Abdalla Ahmed and Paul Delgado-Olguin

10. Isolation and Culture of Mouse Placental Endothelial Cells

Lijun Chi and Paul Delgado-Olguin

11. Flow Cytometry and Lineage Tracing Study for Identification of Adipocyte Precursor Cell (APCs) Populations

Ju Hee Lee, Azadeh Yeganeh, Hisato Konoeda, Joon Ho Moon, and Hoon-Ki Sung

12. Whole-Mount and Section In Situ Hybridization in Mouse Embryos for Detecting mRNA Expression and Localization

Kazuko Koshiba-Takeuchi

13. Chromosome Painting of Mouse Chromosomes

Lisa L. Hua and Takashi Mikawa

14. Chromatin Immunoprecipitation in Early Mouse Embryos

Estela García-González, Bladimir Roque-Ramirez, Carlos Palma-Flores, and J. Manuel

Hernández-Hernández

15. Shaping Up the Embryo: The Role of Genome 3D Organization

Karina Jácome-López and Mayra Furlan-Magaril

16. Genome Edition during Development Using the CRISPR-Cas Technology

Rodrigo Arzate-Mejía, Paula Licona-Limón, and Félix Recillas-Targa

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts