Multipotent Stem Cells of the Hair Follicle, Softcover reprint of the original 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1453

This volume discusses methods for the study of multipotent and pluripotent stem cells of the hair follicle. The stem cells described are involved in both the growth of the hair follicle and its production of the hair shaft, as well as the growth of the hair follicle sensory nerve. Multipotent Stem Cells of the Hair Follicle: Methods and Protocols also explores very unexpected results such as that of the hair follicle-associated-pluripotent (HAP) stem cells, which not only have the capability for regenerating the hair follicle sensory nerve, but also can differentiate ex vivo and in vivo to multiple cell types not associated with the hair follicle?these include glial cells, motor neurons, and beating cardiac muscle cells. The potential for HAP stem cells for regenerative medicine is also discussed in detail. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Multipotent Stem Cells of the Hair Follicle: Methods and Protocols is a valuable resource for researchers interested in this field.

1. Introduction to Hair-Follicle-Associated Pluripotent (HAP) Stem Cells
Robert M. Hoffman

2. Nestin-Based Reporter Transgenic Mouse Lines
John Mignone, Natalia Peunova, and Grigori Enikolopov

3. Discovery of HAP Stem Cells
Lingna Li and Robert M. Hoffman

4. Peripheral-Nerve and Spinal-Cord Regeneration in Mice Using Hair Follicle-Associated-Pluripotent (HAP) Stem Cells
Yasuyuki Amoh, Kensei Katsuoka, and Robert M. Hoffman

5. Construction of Tissue Engineered Nerve Conduits Seeded with Neurons Derived from Hair Follicle Neural Crest Stem Cells
Fang Liu, Haiyan Lin, and Chuansen Zhang

6. Nestin-Expressing Hair Follicle-Associated Pluripotent (HAP) Stem Cells Promote          Whisker Sensory-Nerve Growth in Long-Term 3D-Gelfoam Histoculture
Sumiyuki Mii, Jennifer Duong, Yasunori Tome, Aisada Uchugonova, Fang Liu, Yasuyuki       Amoh, Norimitsu Saito, Kensei Katsuoka, and Robert M. Hoffman

7. Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles
Ruifeng Yang and Xiaowei Xu

8. Isolation of Mouse Hair Follicle Bulge Stem Cells and their Functional Analysis in a   Reconstitution Assay
Ying Zheng, Jen-Chih Hsieh, Julia Escandon, and George Cotsarelis

9. Hair Follicle Regeneration by Transplantation of a Bioengineered Hair Follicle Germ
Katsunari Tezuka, Koh-ei Toyoshima, and Takashi Tsuji

10. Hair Induction by Cultured Mesenchymal Cells Using Sphere Formation
Kazuo Kishi and Ruka Hayashi

11. Stereological Quantification of Cell Cycle Kinetics and Mobilization of Epithelial Stem Cells during Wound Healing
Eduardo Martínez-Martínez, Eileen Uribe-Querol, Claudio I. Galván-Hernández, and Gabriel Gutiérrez-Ospina

12. Culture of Dermal Papilla Cells from Ovine Wool Follicles: An In Vitro Model for Papilla Size-Determination
Nicholas W. Rufaut, Allan J. Nixon, and Rodney D. Sinclair

13. Isolation and Fluorescence Activated Cell-Sorting of Mouse Keratinocytes Expressing β-Galactosidase
Maria Kasper, Rune Toftgård, and Viljar Jaks

14. Ectopic Hair Growth from Transplanted Whisker Follicles on the Spinal Cord of Mice
Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman

15. Long-Term Gelfoam® Histoculture of Hair-Shaft-Producing Mouse Whisker Follicles
Wenluo Cao, Fang Liu, Yasuyuki Amoh, and Robert M. Hoffman

16. From Hair to Heart: Hair Follicle-Associated-Pluripotent (HAP) Stem Cells Differentiate to Beating Cardiac Muscle Cells
Masateru Yashiro, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Robert M. Hoffman, and Yasuyuki Amoh

17. Highly Efficient Neural Differentiation of CD34 Positive Hair Follicle-Associated     Pluripotent (HAP) Stem Cells Induced by Retinoic Acid and Serum Free Medium
Mohsen Sagha and Nowruz Najafzadeh

18. Cryopreservation of Intact Hair Follicle Maintains Pluripotency of Nestin-Expressing (HAP) Stem Cells
Satoshi Kajiura, Sumiyuki Mii, Ryoichi Aki, Yuko Hamada, Nobuko Arakawa, Katsumasa Kawahara, Lingna Li, Kensei Katsuoka, Robert M. Hoffman, and Yasuyuki Amoh         

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts