Myelin, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1791
This volume collects research methodologies for both basic and translational studies of myelin authored by experts in the field. Chapters in this book are divided into three parts: Part One focuses on visualizing myelin and studying it on a biochemical level; Part Two looks at cell culture systems used to uncover key mechanisms that regulate formation and maintenance of myelin; and Part Three discusses modeling disorders of myelin in both CNS and PNS, and therapeutic strategies for targeting these disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Myelin: Methods and Protocols is a valuable resource for physiologists, molecular biologists, anatomists, cell and developmental biologists, and physician-scientists working in the field of myelin biology and the disorders associated with it.
Preface
Table of Contents
Contributing Authors
Part I Imaging Myelin and Biochemical Analysis
1. Electron Microscopy for the Analysis of Peripheral Nerve Myelin
Cinzia Ferri, Angelo Quattrini, and Maurizio D’Antonio
2. Immunohistochemical Analysis of Myelin Structures
M. Thetiot, S.A. Freeman, and A. Desmazières
3. Visualization and Time-Lapse Microscopy of Myelinating Glia In Vivo in Zebrafish
Stavros Vagionitis and Tim Czopka
4. Lipidomics Profiling of Myelin
Chunyan Wang, Juan Pablo Palavicini, and Xianlin Han
5. Mapping Lipid Distribution in Rat Sciatic Nerve Using Imaging Mass Spectrometry
Roberto Fernández, Jone Garate, Beatriz Abad, Begoña Ochoa, and José A. Fernández
Akihiro Ishii, David Han, and Rashmi Bansal
Part II Cell Culture Systems
7. Isolation and Purification of Primary Rodent Schwann Cells
Marta Palomo Irigoyen, Miguel Tamayo Caro, Encarnacion Pérez Andrés, Adrián Barreira Manrique, Marta Varela Rey, and Ashwin Woodhoo
8. Isolation, Expansion, and Maturation of Oligodendrocyte Lineage Cells Obtained from Rat Neonatal Brain and Optic Nerve
Maria Victoria Sánchez-Gómez, Mari Paz Serrano, Elena Alberdi, Fernando Pérez-Cerdá, and Carlos Matute
9. DRG Neuron/Schwann Cells Myelinating Co-Cultures
Carla Taveggia and Alessandra Bolino
10. Neuron/Oligodendrocyte Myelination Co-Culture
Yi Pang, Kimberly Simpson, José Javier Miguel-Hidalgo, and Renate Savich
11. Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination
Mohammad Amin Sherafat, Robert A. Hill, and Akiko Nishiyama12. Modeling PNS and CNS Myelination Using Microfluidic Chambers
Adrien Vaquié, Alizée Sauvain, and Claire Jacob
13. Modeling CNS Myelination Using Micropillar Arrays
Nan-Xing Huang, Yun-An A. Shen, and Feng Mei
14. A Central Nervous System Axonal Myelination Assay for High-Throughput Screening
Karen Lariosa-Willingham and Dmitri Leonoudakis
15. Analyzing Autophagic Flux in Nerve Cultures
Miguel Tamayo Caro, Marta Palomo Irigoyen, Encarnacion Pérez Andrés, Adrián Barreira Manrique, Marta Varela Rey, and Ashwin Woodhoo
16. Subcellular Optogenetic Stimulation Platform for Studying Activity-Dependent Axon Myelination In Vitro
Agata Blasiak, Sudip Nag, and In Hong Yang
Part III Modeling Demyelinating Disorders and In Vivo Targeting Strategies
Mercedes Giralt, Amalia Molinero, and Juan Hidalgo
18. Experimental Demyelination and Remyelination of Murine Spinal Cord by Focal Injection of Lysolecithin
Chenyi Wang and Mark R. Kotter
David G. Gonsalvez, SangWon Yoo, Georgina A. Craig, Rhiannon J. Wood, Jessica L. Fletcher, Simon S. Murray, and Junhua Xiao
20. Transection and Crush Models of Nerve Injury to Measure Repair and Remyelination in Peripheral Nerve
Xin-peng Dun and David B. Parkinson
21. In Vivo Introduction of Transgenes into Mouse Sciatic Nerve Cells Using Viral Vectors
Gerben Van Hameren, Sergio Gonzalez, Ruani N. Fernando, Claire Perrin-Tricaud, and Nicolas Tricaud
22. Intrathecal Delivery of Viral Vectors for Gene Therapy
Alexia Kagiava and Kleopas A. Kleopa
23. Efficient Gene Transfer to Myelinating Schwann Cells in the Rodent Sciatic Nerve Using In Vivo Electroporation
Daisuke Ino and Masamitsu Iino
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Ouvrage de 294 p.
17.8x25.4 cm
Date de parution : 07-2018
Ouvrage de 294 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 179,34 €
Ajouter au panierThème de Myelin :
Mots-clés :
axons; leukodystrophies; neuropathies; Schwann cells; plasma membrane