Description
Neisseria gonorrhoeae, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1997
Coordinator: Christodoulides Myron
Language: EnglishSubjects for Neisseria gonorrhoeae:
Publication date: 08-2020
Support: Print on demand
Publication date: 05-2019
461 p. · 17.8x25.4 cm · Hardback
Description
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General topic area
Chapter title
1
IntroductionIntroduction – biology, disease, pathogenesis
2
Infection and AMR
Algorithms for predicting infection in women
3
Antimicrobial resistance
4
Whole-genome sequencing to predict antibiotic MICs for Ngo
5
?Developing new antimicrobials to gonococci (computer-based? Etc…)
6
GeneticsGenetic transformation of Neisseria gonorrhoeae
7
Transcriptomics for Gc infection
8
RNA-seq for Neisseria gonorrhoeae
9
Biochemistry
Isolation of phenotypic variants by stereo-microscopy
10
Production of peptidoglycan
11
Protein glycosylation in Neisseria gonorrhoeae
12
?Production of purified pili/LPS
13
Vaccine
OMV preparation and characterization
14
Proteomics for vaccine development
15
Reverse vaccinology for Neisseria gonorrhoeae
16
VLP-based vaccines
17
Recombinant protein expression for gonococcal antigens
18
Biological assays
Serum bactericidal assay
19
Mouse intravaginal infection model
20
Human
challenge model21
Mouse CEACAM model of infection humanized mouse model
22
Gonococcal models of eye infection
23
Fallopian tube explant model
24
Neutrophil interactions with Neisseria gonorrhoeae
25
3D endometrial epithelial cell model
26
Rhesus macaque model of Neisseria infection
27
Methods to investigate gonococcal adhesion and invasion of human cells
28
Opsonophagocytosis of N.gonorrhoeae
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts