Neural Crest Cells, 1st ed. 2019
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1976

This detailed book provides a wide range of techniques, from those that have been used extensively since the very first investigations into neural crest cells to those that are currently cutting-edge, in order to explore the development of neural crest cells in human, mice, rat, chick, quail, medaka, and shark. With a bit of imagination and adjustment, many of these methodologies can be adaptable to any species desirable for study. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

1.         The neural crest: a remarkable model system for studying development and disease (Chaya Kalcheim)

2.         Addressing interdisciplinary difficulties in developmental biology / mathematical collaborations: a neural crest example (Donald Newgreen, Kerry Landman and James Osborne)

3.         Generation of neural crest progenitors from human pluripotent stem cells (Kwaku Dad Abu-Bonsrah, Serena Viventi, Donald Newgreen and Mirella Dottori)

5.         Deciphering the neural crest contribution to cephalic development with avian embryos (Moussab Alrajeh, Zuzana Vavrusova and Sophie Creuzet)

6.         In ovo electroporation of plasmid DNA and morpholinos into specific tissues during early embryogenesis (Rebecca McLennan and Paul Kulesa)

7.         Ex vivo culture and manipulation of mouse neural crest cells from primary embryonic tissue explants (Sophie Wiszniak)

8.         Technologies for live imaging of enteric neural crest derived cells (Marlene Hao, Annette Bergner, Donald F. Newgreen, Hideki Enomoto and Heather Young)

9.         Mouse embryo culture for the study of neural crest cells (William Munoz and Paul Trainor)

10.       Laser capture microdissection of murine embryonic neural crest cells (Robert Greene, Irina Smolenkova and Michele Pisano)

11.       In vivo and in vitro quantitative analysis of neural crest cell migration (Elias Barriga, Adam Shellard and Roberto Mayor)

12.       Proteomics in neural crest cell research (Peter McCarthy and Quenten Schwarz)

13.       Neural crest methodologies in zebrafish and medaka (Kleio Petratou, Karen Camargo-Sosa, Ruqaiya Al Jabri, Yusuke Nagao and Robert Kelsh)

14.       Fluorescence-activated cell sorting and nanostring profiling of single neural crest cells and pigment cells (Tatiana Subkhankulova and Robert Kelsh)

15.       Live imaging of neural crest and pigment cells and transient transgenic manipulation of gene activity (Reyna Deeya Ballim, Yusuke Nagao and Robert Kelsh)

16.       Avenues for investigating the neural crest and its derivatives in non-model (unconventional) vertebrates: a craniofacial skeleton perspective (Micheal Depew and Federica Bertocchini)

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts