Peptide Nucleic Acids (3^rd Ed., 3rd ed. 2020) Methods and Protocols Methods in Molecular Biology Series, Vol. 2105

This third volume exemplifies and illustrates exciting advances in peptide nucleic acids (PNA) chemistry, and serves as a vital complement to the first and second edition of the book.  Chapters focus on in vivo properties and behavior and applications of PNA while providing contributions on both chemistry and nucleic acid recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Peptide Nucleic Acid: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

1. Fmoc-based Assembly of PNA Oligomers: Manual and Microwave-assisted Automated Synthesis

Ashif Yasin Shaikh, Anna Mette Hansen, and Henrik Franzyk

2. A Robust Method for Preparing Optically Pure MiniPEG-Containing Gamma PNA Monomers

Wei-Che Hsieh and Danith H. Ly

3. Synthesis of Pyrrolidinyl PNA and Its Site-specific Labeling at Internal Positions by Click Chemistry

Boonsong Ditmangklo, Penthip Muangkaew, Kotchakorn Supabowornsathit, and Tirayut Vilaivan

4. Synthesis of PNA-peptide Conjugates as Functional SNARE Protein Mimetics

5. Lipid-modified Peptide Nucleic Acids – Synthesis and Application to Programmable Liposome Fusion

6. Facile Preparation of PNA-Peptide Conjugates with a Polar Maleimido-thioether Linkage 

7. PNA-Encoded Synthesis (PES) and DNA Display of Small Molecule Libraries

8. Transcription Inhibition by PNA-induced R-loops

Boris P. Belotserkovskii, Sum-yan Ng, and Philip C. Hanawalt

9. Nucleobase-Modified Triplex-Forming Peptide Nucleic Acids for Sequence-Specific Recognition of Double-Stranded RNA

Nikita Brodyagin, Dziyana Hnedzko, James A MacKay, and Eriks Rozners

10. In Vitro Cellular Delivery of Peptide Nucleic Acid (PNA)

Takehiko Shiraishi, Mahdi Ghavami, and Peter E. Nielsen

11. Reactive Quantum Dot-based FRET Systems for Target-catalyzed Detection of RNA

Oleksandr Zavoiura, Ute Resch-Genger, and Oliver Seitz

12. Peptide Nucleic Acids for MicroRNAs Targeting

Roberto Gambari, Jessica Gasparello, Enrica Fabbri^, Monica Borgatti, Anna Tamanini, and Alessia Finotti

13. Detection of Microorganisms by Fluorescence in situ Hybridization using Peptide Nucleic Acid

Ricardo Oliveira, Carina Almeida, and Nuno F. Azevedo

14. PNA Antisense Targeting in Bacteria. Determination of Antibacterial Activity (MIC) of PNA-peptide Conjugates

Lise Goltermann and Peter E. Nielsen

Camilla Brolin, Ernest Wee Kiat Lim, and Peter E. Nielsen

17. Poly(lactic-co-glycolic acid) Nanoparticle Delivery of Peptide Nucleic Acids In Vivo

Stanley N. Oyaghire, Elias Quijano, Alexandra S. Piotrowski-Daspit, W. Mark Saltzman, and Peter M. Glazer

18. Preparation of Conjugates for Affibody-based PNA-mediated Pretargeting

Mohamed Altai, Anzhelika Vorobyeva, Vladimir Tolmachev, Amelie Eriksson Karlström, and Kristina Westerlund

Includes cutting-edge methods and Protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts