Phosphoinositides, 1st ed. 2021 Methods and Protocols Methods in Molecular Biology Series, Vol. 2251

1. Simultaneous Detection of Phosphoinositide Lipids by Radioactive Metabolic Labeling

            Noah Steinfeld, Sai Srinivas Panapakkam Giridharan, Emily J. Kauffman, and Lois S. Weisman

2. Label-Free Quantification of Phosphoinositides in Drosophila by Mass Spectrometry

            Avishek Ghosh and Padinjat Raghu

3. Profiling of Phosphoinositide Molecular Species in Resting or Activated Human or Mouse Platelets by a LC-MS Method

            Gaëtan Chicanne, Justine Bertrand-Michel, Julien Viaud, Karim Hnia, Jonathan Clark, and Bernard Payrastre

4. Quantification of Genetically-Encoded Lipid Biosensors

            Rachel C. Wills, Jonathan Pacheco, and Gerald R.V. Hammond

5. Detection of Plasma Membrane Phosphoinositide Dynamics Using Genetically-Encoded Fluorescent Protein Probes

            Rebecca Cabral-Dias, Yasmin Awadeh, Roberto J. Botelho, and Costin N. Antonescu

6. Imaging the Nanoscale Distribution of Phosphoinositides in the Cell Plasma Membrane with Single-Molecule Localization Super-Resolution Microscopy

            Fan Fan, Chen Ji, and Xuelin Lou

7. Induced Dimerization Tools to Deplete Specific Phosphatidylinositol Phosphates

            Jonathan Pacheco, Rachel C. Wills, and Gerald R.V. Hammond

8. A Real-Time Phosphatidylinositol 4-Phosphate 5-Kinase Assay Using Fluorescence Spectroscopy

            Taki Nishimura

9. Assessing In Situ Phosphoinositide-Protein Interactions through Fluorescence Proximity Ligation Assay in Cultured Cells

            Mo Chen, Hudson T. Horn, Tianmu Wen, Vincent L. Cryns, and Richard A. Anderson

10. Characterization of Protein-Phospholipid/Membrane Interactions Using a “Membrane-on-a-Chip” Microfluidic System

            Calvin Yeager, Djoshkun Shengjuler, Simou Sun, Paul S. Cremer, and Craig E. Cameron

11. Exploring Phosphoinositide Binding Using Native Mass Spectrometry

            Julian Bender and Carla Schmidt

12. Liposome-Based Methods to Study Protein-Phosphoinositide Interaction

            Mélanie Mansat, Mélanie Picot, Gaëtan Chicanne, Virginie Nahoum, Frédérique Gaits-Iacovoni, Bernard Payrastre, Karim Hnia, and Julien Viaud

13. Liposome-Based Methods to Study GTPases Activation by Phosphoinositides

            Julien Viaud, Laurie Ceccato, Bernard Payrastre, and Frédérique Gaits-Iacovoni

14. Liposome Co-Sedimentation and Co-Flotation Assays to Study Lipid-Protein Interactions

            Yosuke Senju, Pekka Lappalainen, and Hongxia Zhao

15. Characterization of PROPPIN-Phosphoinositide Binding by Stopped-Flow Fluorescence Spectroscopy

            Ángel Pérez-Lara and Reinhard Jahn

16. Fluorescence Assays to Study Membrane Penetration of Proteins

            Yosuke Senju and Hongxia Zhao

17. Fluorogenic XY-69 in Lipid Vesicles for Measuring Activity of Phospholipase C Isozymes

            Adam J. Carr, Edhriz Siraliev-Perez, Weigang Huang, John Sondek, and Qisheng Zhang

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts