Programmed Necrosis, Softcover reprint of the original 1st ed. 2018
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1857

This volume explores the latest techniques and approaches used to study this emerging form of cell death. The chapters in this book cover topics such as distinguishing necroptosis from apoptosis; detecting MLKL oligomerization during programmed necrosis; analyzing the RIPK3 necrosome complex; using RIPK1 kinase small molecule inhibitors in studying necroptosis; monitoring RIPK1 phosphorylation in the TNFR1 signaling complex; and assessing in vivo kidney cell death in glomerular injury. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Programmed Necrosis: Methods and Protocols is a valuable resource for technicians, graduate students, postdoctoral fellows, and other scientists who are pursuing studies in this field.

Preface…

Table of Contents…

Contributing Authors…

1. Tools in the Art of Studying Necroptosis

Adrian T. Ting

2. Loss-of-Function RNAi Screen to Identify Necrosis-Signaling Molecules

David Mark Moquin and Francis Ka-Ming Chan

3. Chemical Library Screens to Identify Pharmacological Modulators of Necroptosis

Danish Saleh and Alexei Degterev

4. Distinguishing Necroptosis from Apoptosis

Inbar Shlomovitz, Sefi Zargarian, Ziv Erlich, Liat Edry-Botzer, and Motti Gerlic

5. Methods for Studying TNF-Mediated Necroptosis in Cultured Cells

Zikou Liu, John Silke, and Joanne M. Hildebrand

6. Analysis of Necroptosis in Bone Marrow-Derived Macrophages

Diana Legarda and Adrian T. Ting

Diego A. Rodriguez and Douglas R. Green

8. Detection of MLKL Oligomerization during Programmed Necrosis

Zhenyu Cai and Zheng-Gang Liu

9. Analysis of Cytokine- and Influenza A Virus-Driven RIPK3 Necrosome Formation

Roshan J. Thapa, Shoko Nogusa, and Siddharth Balachandran

10. Detection of RIPK1 in the FADD-Containing Death Inducing Signaling Complex (DISC) during Necroptosis

Rosalind L. Ang and Adrian T. Ting

11. Use of RIP1 Kinase Small Molecule Inhibitors in Studying Necroptosis

Allison M. Beal, John Bertin, and Michael A. Reilly

12. Analyzing Necroptosis using a RIPK1 Kinase Inactive Mouse Model of TNF Shock

Matija Zelic and Michelle A. Kelliher

13. Assessment of In Vivo Kidney Cell Death: Acute Kidney Injury

Wulf Tonnus, Moath Al-Mekhlafi, Christian Hugo, and Andreas Linkermann

14. Assessment of In Vivo Kidney Cell Death: Glomerular Injury

Wulf Tonnus, Moath Al-Mekhlafi, Florian Gembardt, Christian Hugo, and Andreas Linkermann

15. Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling

Joshua D. Webster, Margaret Solon, Susan Haller, and Kim Newton

Matthias Reichert, Amandeep Bhamra, Sebastian Kupka, and Henning Walczak

17. Monitoring RIPK1 Phosphorylation in the TNFR1 Signaling Complex

Dario Priem, Yves Dondelinger, and Mathieu JM Bertrand

Diana Legarda and Adrian T. Ting

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts